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Yeast Colony Embedding Method
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酵母细胞真空注入真菌颗粒作为一种新的细胞封装方法
Lara Lúquez-Caravaca1, Minami Ogawa1,2, Rewa Rai2
1Department of Agricultural Chemistry, Edaphology and Microbiology, University of Córdoba, 14014, Córdoba, Spain.
Applied microbiology and biotechnology
|July 25, 2023
概括
一种新的方法使真菌颗粒中的酵母不动,显著提高生产率和葡萄酒制作的细胞保留率. 这种改进的酵母生物囊技术保持了细胞功能,并为各种工业应用提供了潜力.
科学领域:
- 生物技术是生物技术.
- 食品科学 食品科学 食品科学
- 微生物学 微生物学
背景情况:
- 动不动的酵母细胞在酒中至关重要,特别是在泡和雪利葡萄酒中.
- 现有的酵母固定方法,如共同培养,在生产力和细胞保留方面存在局限性.
研究的目的:
- 开发和评估一种用于将酵母细胞固定在线状真菌颗粒中的新方法.
- 将这种新方法与以前用于酵母生物囊生产和性能的技术进行比较.
主要方法:
- 酵母细胞被输入到真空应用的真菌颗粒中,然后在YPD介质中促进生长.
- 这种新方法与以前的共同培养方法进行了比较,该方法使用葡萄末的酒精发酵.
- 评估包括生物囊生产率,细胞保留,细胞生物活性和葡萄酒质量分析.
主要成果:
- 新的固定化技术使生物囊的生产率提高了37.40倍.
- 生物囊中的细胞保留率提高了三倍,发酵期间细胞泄漏率降低了两倍.
- 虽然大多数葡萄酒的化学特征与传统生产的葡萄酒相似,但感官分析显示了香味,酸度,苦味和持久性的积极差异.
结论:
- 新的真空辅助输液和生长方法显著提高了酵母生物囊的生产力和细胞保留.
- 固定化的酵母细胞保留了酒精发酵的功能,产生具有理想感官特征的葡萄酒.
- 这种多功能封装技术对葡萄酒制造以外的更广泛的工业应用具有前景.
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