使用化S-腺-l-氨酸辅因子进行生物催化基化
Wenping Ding1,2, Minqi Zhou3,4, Huayu Li2
1Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, International Joint Research Center for Intelligent Biosensor Technology and Health, College of Chemistry, Central China Normal University, Wuhan 430079, China.
Organic letters
|July 25, 2023
概括
这项研究提出了一种用于生物催化醇基化的新型多酶方法. 该策略使复杂分子如万科米辛的选择性化成为可能,从而推动了药物开发.
科学领域:
- 有机化学 有机化学
- 生物催化剂是一种生物催化剂.
- 药用化学 医学化学
背景情况:
- 的结合对于制药的发展至关重要.
- 生物催化方法为化学合成提供了可持续的替代方案.
研究的目的:
- 开发生物催化基化多酶策略.
- 为了使复杂分子的晚期选择性化.
主要方法:
- 使用了依赖S-adenosyl-l-methionine (SAM) 的甲基转移酶 (MTs).
- 制备的化SAM辅因子,使用工程人甲氨基基转移酶hMAT2AI322A.
- 将该策略应用于晚期修改的万科米辛.
主要成果:
- 实现了第一个生物催化3,3-difluoroallylation的例子.
- 在晚期基化范科米中,已证明高转化率 (高达99%) .
- 展示了多酶方法的多功能性.
结论:
- 开发的多酶策略对生物催化醇基化有效.
- 这种方法促进复杂的药物化合物的选择性化.
- 为药物发现和开发提供了一个有前途的工具.
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