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黄金鱼 (Carassius auratus L.) 两种颗粒细胞殖民地刺激因子的分子和功能表征

Moussa Gouife¹, Feiyang Wang², Ziqi Ban³

¹Zhejiang Institute of Freshwater Fisheries, Huzhou, Zhejiang 313001, China; School of Marine Sciences, Ningbo University, Ningbo, Zhejiang 315211, China. Electronic address: https://twitter.com/Mouskharim.

Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology

|July 25, 2023

概括

金鱼颗粒细胞殖民地刺激因子 (GCSF-a和GCSF-b) 调节中性粒细胞的发育和免疫反应. 这些因素刺激白细胞的增殖和分化,影响造血转录因子和细胞因子的产生.

科学领域:

免疫学免疫学免疫学
分子生物学分子生物学
鱼类生物学鱼类生物学

背景情况:

颗粒细胞殖民地刺激因子 (GCSF) 对于脊椎动物中性粒细胞的发育至关重要.
有限的信息存在于GCSF功能在teleost造血.
这项研究研究了两种金鱼GCSF基因,gfGCSF-a和gfGCSF-b.

研究的目的:

在分子和功能上描述两个金鱼GCSF基因.
阐明gfGCSF-a和gfGCSF-b在金鱼血液形成和免疫反应中的作用.

主要方法:

分子克隆和gfGCSF-a和gfGCSF-bcDNA的测序.
用LPS和Aeromonas hydrophila刺激的金鱼头白细胞 (HKLs) 中gfGCSF表达的分析.
在HKLs上使用重组蛋白 (rgGCSF-a和rgGCSF-b) 的体外功能测定.

主要成果:

gfGCSF-a和gfGCSF-b的mRNA水平被LPS和A.水菌暴露上调.
再组合的gfGCSF诱导了来自中性粒细胞的剂量依赖的TNFα和IL-1β产生.
rgGCSF-a和rgGCSF-b差异地促进白细胞的增殖和分化,影响关键的造血转录因子.

结论:

金鱼GCSF-a和GCSF-b是中性粒细胞增殖和分化的关键调节者.
这些因素在刺激鱼类血液形成的各个阶段和谱系中起着重要作用.
这些发现有助于理解GCSF在远程免疫和造血系统中的功能.

关键词:

细胞因子细胞因子鱼类鱼类鱼类鱼类鱼类这是GCSF的GCSF.血液形成血液形成血液形成作为一个中性粒细胞.

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