介质干细胞衍生的外体减弱了由西斯普拉丁和白素诱导的DNA损伤反应
Xiaoqiang Hu1, Chuncao He2, Lijun Zhang2
1Department of Occupational and Environmental Health, Hangzhou Normal University School of Public Health, Hangzhou 311121, China.
概括
人类胚胎干细胞衍生的外体细胞可以保护DNA免受cisplatin和bleomycin的损伤. 这些干细胞衍生外体 (SC-Exos) 减少活性氧物种,为细胞提供对基因毒剂的保护.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 生物技术是生物技术.
背景情况:
- 来自干细胞的外生体 (SC-Exos) 显示出保护细胞免受DNA损伤的潜力.
- 之前的研究还没有系统地比较对不同类型的DNA损伤的外体细胞疗效.
研究的目的:
- 评估人类胚胎干细胞诱导的介质干细胞衍生外体 (hESC-MSC-Exos) 对两种不同的DNA损伤剂的保护作用.
- 为了比较hESC-MSC-Exos在减轻cisplatin (线程内/线程间交叉连接) 和bleomycin (双线程断裂) 引起的DNA损伤方面的疗效.
主要方法:
- 利用性彗星试验量化了用西斯或白素治疗的HeLa细胞中的DNA损伤,有或没有hESC-MSC-Exos.
- 通过免疫光和西部涂抹来评估基因组H2AX (γH2AX) 的表达.
- 测量了细胞内活性氧物种 (ROS) 水平,使用二二二化二酸盐染色.
主要成果:
- hESC-MSC-Exos显著降低了DNA损伤标记 (尾巴DNA百分比,尾巴时刻,橄尾巴时刻) 在西斯普拉丁和白胺治疗时以剂量依赖的方式.
- 使用hESC-MSC-Exos的预处理导致γH2AX表达减少了50%以上,这表明减少了DNA双链断裂.
- hESC-MSC-Exos有效地抑制了药物诱导的细胞内ROS的增加.
结论:
- hESC-MSC-Exos对细胞提供了显著的保护,防止由西斯和白素诱导的DNA损伤.
- 保护机制涉及细胞内活性氧物种 (ROS) 的抑制.
- 这些发现突显了hESC-MSC-Exos在减轻基因毒性的治疗潜力.
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