在性大动脉疾病中,circRNA/lncRNA-miRNA-mRNA网络和基因景观
Yuqi Zheng1,2,3, Shuyu Wen4, Shijiu Jiang1,2,3,5
1Department of Cardiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.
BMC genomics
|July 25, 2023
概括
研究人员确定了一种新的circRNA/lncRNA-miRNA-mRNA网络,用于动脉疾病 (CAVD). 这项研究为CAVD提供了潜在的诊断标志物和治疗点,这是一种常见的心脏膜疾病.
科学领域:
- 心血管生物学 心血管生物学
- 分子遗传学 分子遗传学
- 在RNA生物学,RNA生物学.
背景情况:
- 动脉疾病 (CAVD) 是一种流行的心脏门疾病,发病率越来越高,目前没有药物治疗.
- 非编码RNAs,包括循环RNAs (circRNAs) 和长非编码RNAs (lncRNAs),都与CAVD病原发生有关.
- 了解涉及这些分子的复杂调节网络以及它们与微RNA (miRNA) 和信使RNA (mRNA) 的相互作用,对于开发有效疗法至关重要.
研究的目的:
- 通过构建一个circRNA/lncRNA-miRNA-mRNA相互作用网络来阐明CAVD背后的分子机制.
- 确定关键的差异表达基因 (DEG) 和参与CAVD.的调节途径.
- 根据细胞类型 (膜间歇细胞,VIC;膜内皮细胞,VEC),叶片表型和CAVD.中的性别差异进行子组分析.
主要方法:
- 在CAVD中对circRNAs,lncRNAs,miRNAs和mRNAs的差异表达分析.
- 使用定量实时PCR (qRT-PCR) 构建和验证一个circRNA/lncRNA-miRNA-mRNA相互作用网络.
- 在特定细胞类型,叶片表型和性别中识别DEG;对相关途径和免疫细胞模式进行丰富分析.
主要成果:
- 158个DEcircRNAs,397个DElncRNAs,45个DEmiRNAs和167个DEmRNAs的识别.
- 建立一个经过验证的监管网络,涉及hsa-circ-0073813/hsa-circ-0027587-hsa-miR-525-5p-SPP1/HMOX1/CD28.
- 在VICs (17),VECs (9),传单表型 (7) 和性别 (24) 之间发现了特定的DEGs;丰富分析突出显示了炎症和纤维化通路,M0巨细胞和记忆B细胞增加.
结论:
- 已建立的circRNA/lncRNA-miRNA-mRNA轴为CAVD机制提供了新的见解.
- 与CAVD特征相关的已识别的DEG提供了潜在的诊断生物标志物.
- 这项研究为未来的CAVD干预提供了有希望的治疗目标.
相关概念视频
Coronary Artery Disease II: Pathophysiology
13
Coronary Artery Disease (CAD) originates from a series of events that impair the function of coronary arteries, the blood vessels responsible for delivering oxygen-rich blood to the heart muscle. The pathophysiology of CAD is closely linked to atherosclerosis, a chronic inflammatory and lipid-driven condition affecting the vascular endothelium.1. Endothelial DamageThe process begins with damage to the vascular endothelium, which serves as a protective barrier between the blood and the vessel...
13
Aortic Regurgitation I: Introduction
13
IntroductionAortic regurgitation is characterized by the backward flow of blood from the aorta into the left ventricle during diastole and arises from the improper closure of the aortic valve. This condition results in left ventricular volume overload and can stem from both acute and chronic etiologies, each contributing uniquely to the disease's progression and symptomatology.Acute and Chronic CausesAcute aortic regurgitation often results from events that suddenly impair the integrity of the...
13
Imaging Studies for Cardiovascular System VI: Calcium -Scoring CT
41
Calcium-Scoring CT ScanA calcium-scoring CT scan, also known as coronary artery calcium (CAC) scan, detects calcium deposits in the coronary arteries. This test assesses the risk of coronary artery disease (CAD), which can lead to cardiovascular events such as angina, heart failure, and sudden cardiac arrest.A calcium-scoring CT scan is generally recommended for individuals at intermediate risk of CAD without symptoms. It includes:Men aged 40-75 and women aged 50-75: Especially those with a...
41
Aortic Regurgitation III: Medical Management
25
Aortic regurgitation (AR) is when the aortic valve does not close or seal properly, leading to backward blood circulation from the aorta into the left ventricle during diastole. Common causes of AR include rheumatic heart disease, congenital valve defects, and aortic root dilation. Managing AR requires a multifaceted approach to alleviate symptoms, preserve left ventricular function, and address the underlying cause of the regurgitation. Patients with symptomatic AR or significant left...
25
lncRNA - Long Non-coding RNAs
8.6K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.6K
Mitral Valve Prolapse I: Introduction
22
IntroductionThe mitral valve, one of the heart's four valves, regulates blood flow. These valves have flaps that open and close to direct blood properly through the heart and body. During each heartbeat, the flaps open for blood to pass through and seal shut to prevent backflow. Specifically, the mitral valve opens to allow blood flow from the heart's upper left chamber to the lower left chamber. It then closes securely as the lower left chamber contracts to pump blood to the body, preventing...
22


