相关实验视频

Updated: Jul 21, 2025

Induction and Diverse Assessment Indicators of Experimental Autoimmune Encephalomyelitis

Induction and Diverse Assessment Indicators of Experimental Autoimmune Encephalomyelitis

Published on: September 9, 2022

铁亡诱导慢性EAE的有害影响及其对渐进性MS的影响

Priya Jhelum¹, Stephanie Zandee^2,3, Fari Ryan¹

¹Centre for Research in Neuroscience and BRaIN Program, Research Institute of the McGill University Health Centre (RI-MUHC), Livingston Hall, Room L7-210, 1650 Cedar Ave., Montreal, QC, H3G 1A4, Canada.

Acta neuropathologica communications

|July 25, 2023

概括

铁亡是一种细胞死亡途径,涉及铁和脂质过氧化,涉及慢性实验性自身免疫脑膜炎 (CH-EAE) 和继发性渐进性多发性硬化症 (SPMS). 抑制铁亡改善了CH-EAE结果,表明其治疗潜力.

科学领域:

神经科学是一个神经科学.
免疫学免疫学免疫学
细胞生物学细胞生物学

背景情况:

铁亡是一种受调节的细胞死亡途径,由依赖铁的脂质过氧化驱动.
氧化应激和铁沉积与渐进的多发性硬化症 (MS) 有关.
实验性自身免疫脑膜炎 (EAE) 模型被用来研究多发性硬化症的发病过程.

研究的目的:

为了研究铁死在慢性EAE (CH-EAE) 中的作用,MS的小鼠模型.
检查CH-EAE和人类SPMS中的铁灭标志物和治疗干预措施.

主要方法:

在CH-EAE中评估了与ferroptosis相关的基因和蛋白质的mRNA和蛋白质水平.
分析了铁代谢标记物,抗氧化途径和脂质过氧化.
用铁灭抑制剂治疗的CH-EAE小鼠.
检查了SPMS患者的尸检组织.

主要成果:

CH-EAE显示出改变了铁灭菌基因表达和增加了铁进口物,血红素氧酶-1和ferritin.
核受体协活性剂4 (NCOA4) 的表达增加,与氧化还原活性铁和脂质过氧化相关.
在CH-EAE中,抗氧化途径减少.
铁灭抑制改善了CH-EAE功能和病理学.
在SPMS组织中,铁载细胞附近的巨细胞和寡细胞中显示出NCOA4的表达.

结论:

铁死在CH-EAE的发病过程中起着重要作用.
通过NCOA4介导的费里替诺法基有助于CH-EAE和SPMS中的铁释放和铁.
向铁亡可能为渐进性MS提供治疗策略.

关键词:

抗氧化剂抗氧化剂是一种抗氧化剂.实验性自身免疫性脑膜炎铁的毒性铁的毒性脂质过氧化是一种过氧化.没有NCOA4的二次渐进性MSMS的第二阶段.

更多相关视频

Author Spotlight: Creating a Versatile Experimental Autoimmune Encephalomyelitis Model Relevant for Both Male and Female Mice

Author Spotlight: Creating a Versatile Experimental Autoimmune Encephalomyelitis Model Relevant for Both Male and Female Mice

Published on: October 13, 2023

Implanting Glass Spinal Cord Windows in Adult Mice with Experimental Autoimmune Encephalomyelitis

Implanting Glass Spinal Cord Windows in Adult Mice with Experimental Autoimmune Encephalomyelitis

Published on: December 21, 2013

相关概念视频

Necrosis

Necrosis

Necrosis is considered as an “accidental” or unexpected form of cell death that ends in cell lysis. The first noticeable mention of “necrosis” was in 1859 when Rudolf Virchow used this term to describe advanced tissue breakdown in his compilation titled “Cell Pathology”.
Morphological Manifestations of Necrosis
Necrotic cells show different types of morphological appearance depending on the type of tissue and infection. In coagulative necrosis, cells become...