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TARPγ2 对于哺乳动物视网膜的方向选择电路中的正常AMPA受体表达和功能是必需的

Todd Stincic¹, Jacqueline Gayet-Primo^2,3,1, W Rowland Taylor^2,3,1

¹Casey Eye Institute, Oregon Health and Science University, Portland, OR 97239.

|July 25, 2023

概括

跨膜AMPA受体调节蛋白gamma2 (TARPγ2) 对于视网膜中AMPA受体 (AMPARs) 的突触表达至关重要. 在星光观察者小鼠中,TARPγ2的丧失会减少AMPARs,并改变视网膜电路功能.

科学领域:

神经科学是一个神经科学.
视网膜生理学视网膜生理学
分子生物学分子生物学

背景情况:

AMPA受体 (AMPARs) 在视网膜中调解快速刺激性神经传递.
辅助子单元,包括TARP,调节AMPAR功能,但视网膜TARP的组成是未知的.
星爆亚马克林细胞 (SAC) 是视网膜处理中的关键内部神经元.

研究的目的:

为了确定视网膜中的TARP子单元及其在AMPAR调节中的作用.
调查TARPγ2 (星加) 在视网膜突触传输中的功能.
分析TARPγ2缺乏对视网膜电路功能和视觉处理的影响.

主要方法:

在老鼠,子,和人类视网膜中的免疫组织化学.
鼠标和人类SAC的转录组分析.
从星 (TARPγ2淘汰赛) 小鼠的视网膜切片上的电生理学.
在ON-SACs和方向选择性结节细胞中分析光唤起的反应.

主要成果:

TARPγ2定位在哺乳动物视网膜的突触层中,特别是在SACs分支的地方.
星老鼠的TARPγ2缺乏症显著降低了突触GluA2,GluA3和GluA4的表达.
星鼠中的ON-SACs表现出较小的AMPAR介导的EPSC和改变的GABAergic输入.
在星光观察者小鼠中,方向选择性质细胞显示了减少的环绕抑制.

结论:

在内视网膜中,TARPγ2对于特定AMPAR亚单元 (GluA2,GluA3,GluA4) 的突触表达至关重要.
缺少TARPγ2会损害视网膜电路功能,影响SAC和视觉空间调.
星突变体中的残留AMPAR表达表明其他TARP子单元的潜在补偿作用.

关键词:

这是一个AMPAPAAMPA.塔普是一个TARP.质细胞是质细胞.视网膜视网膜视网膜是一个星星爆发的亚马克林细胞

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