空间激活的保护性奥克辛转录因子调节模块控制了大米中的新根器官生成
Tushar Garg1,2, Manoj Yadav1,3, Khrang Khrang Khunggur Mushahary1
1Department of Biosciences and Bioengineering, Indian Institute of Technology, Roorkee, Uttarakhand, 247667, India.
Planta
|July 25, 2023
概括
冠根的发育和体外根的再生利用了一个共享的auxin-OsWOX10通路. 这一发现澄清了大米植物和组织培养过程中新根启动的分子基础.
科学领域:
- 植物生物学 植物生物学
- 发展生物学 发展生物学
- 分子遗传学 分子遗传学
背景情况:
- 米具有纤维根系,其中冠根 (CR) 从芽组织中发展.
- 有效的新根启动需要强烈的辅酶反应和冠状根原生植物 (CRP) 建立下游途径的激活.
- 在米在体外再生过程中空间受限的根器官生成的机制尚未完全理解.
研究的目的:
- 在体内幼苗再生过程中,研究素诱导的米新型根器官生成的基础分子机制.
- 确定在自然冠根发育和体外根形成之间共享的共同监管模块.
- 为了阐明OsWOX10在大米根器官生成中的作用.
主要方法:
- 全球基因表达数据集的比较分析.
- 关键基因的时空表达模式分析.
- 米的功能分析 WUSCHEL-RELATED HOMEOBOX 10 (OsWOX10) 在米和Arabidopsis.
主要成果:
- 确定了在CRP启动和脱落过程中参与auxin稳态和信号传递的基因.
- 发现外源性auxin在空间定义的CRP中诱导特定的转录因子.
- 证明OsWOX10促进了大米中的 de novo 根器官生成,并诱导了Arabidopsis中的偶然根.
结论:
- 一个常见的auxin-OsWOX10调节模块控制了大米中的自然冠根发育和auxin诱导的de novo根器官生成.
- 这种调节模块是保存和功能,在诱导偶然的根在异质系统,如Arabidopsis.
- 这些发现揭示了植物在不同条件下根器官生成的基本机制.
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