在Shewanella oneidensis中探测细胞质过氧化物代谢
Yael Toporek1, David Pak1, Hannah Snyder1
1School of Biological Sciences, Georgia Institute of Technology, Atlanta, GA 30332, United States.
FEMS microbiology letters
|July 26, 2023
概括
雪瓦尼拉 (Shewanella oneidensis) 的呼吸会导致反应性氧物种. 这项研究使用HyPer-3传感器揭示了细胞质过氧化物水平是由特定的酶控制的,包括KatG2.
科学领域:
- 微生物学 微生物学
- 生物化学 生物化学
- 细胞还氧化剂恒常状态 细胞还氧化剂恒常状态
背景情况:
- 谢瓦内拉 (Shewanella oneidensis) 是一种具有多样性电子受体的有机无气体.
- 在S.oneidensis中,呼吸可以导致电子泄漏和产生反应性氧物种,如过氧化 (H2O2).
- 呼吸对细胞质氧化还原平衡的影响尚不清楚.
研究的目的:
- 为了研究shewanella oneidensis中的细胞质过氧化 (H2O2) 水平.
- 描述特定过氧化物清除酶在维护氧化还原稳态中的作用.
- 使用HyPer-3 H2O2传感器进行细菌细胞质的体内检查.
主要方法:
- 在野生型和突变菌株Shewanella oneidensis中部署H2O2传感器HyPer-3.
- 在S.oneidensis野生型菌株中验证HyPer-3传感器信号.
- 对缺少关键过氧化物清除酶的基因删除突变体中细胞质H2O2度的分析.
主要成果:
- 在S. oneidensis. 中,HyPer-3传感器显示了0-250μM H2O2的动态范围.
- 缺乏周等离子体谷氨过氧化酶 (PgpD) 和催化酶 (KatB,KatG1,KatG2) 的突变菌株表现出高的细胞质H2O2水平.
- 突变体中高细胞质H2O2度与减少的H2O2去除率相关.
结论:
- 这项研究提供了使用HyPer-3传感器对S. oneidensis细胞质的氧化还原环境的第一个体内分析.
- 在S.oneidensis中,氧回归稳定是通过周等离子体PgpD和细胞质 katalases/peroxidases (KatB,KatG1,KatG2) 的联合作用来维持的.
- 细胞质酶KatG2在过氧化物排毒中发挥着重要的,以前被忽视的作用.
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