使用金属阴离子添加物和高分辨率离子移动性质谱法,改善了芬太尼异构体的分离
Ralph Aderorho1, Christopher D Chouinard1
1Department of Chemistry, Clemson University, Clemson, SC, USA.
Drug testing and analysis
|July 26, 2023
概括
这项研究开发了一种快速离子流动性质谱法,用于检测和分离芬太尼异构体. 改进的技术提高了法医和临床分析的识别准确性,有助于打击阿片类药物滥用.
科学领域:
- 法医化学 法医化学
- 分析化学 分析化学
- 质谱测量质量谱测量
背景情况:
- 滥用芬太尼和致命事故需要先进的分析方法来识别异构体.
- 现有的方法难以区分各种芬太尼类同类物和异构物.
- 准确的检测对于法医和临床毒理学至关重要.
研究的目的:
- 开发一种快速离子流动性质谱法 (IM-MS) 方法,用于芬太尼异构体的分离和检测.
- 通过使用非传统的引证和先进的数据处理来增强差异化策略.
- 建立一种可靠的方法来量化生物样本中的芬太尼.
主要方法:
- 利用离子移动性质谱法 (IM-MS) 根据大小,形状和电荷来分离离子.
- 采用了非常规的阴子添加物 (和过渡金属) 来改善离子分化.
- 实现了高分辨率去复杂化,用于数据后处理,以增加分辨率.
- 收集了各种金属离子添加物的碰撞横截面 (CCS) 值.
主要成果:
- 通过使用[M+Cu]+和[M+Ag]+添加物,在芬太尼尔异构体之间的分辨率得到了显著改善.
- 高分辨率后处理将分辨率提高到>150,而标准仪器的分辨率为50-60.
- 在人类尿液中成功检测和量化芬太尼,检测极限 (LOD) 为13 pg/mL,量化极限 (LOQ) 为40 pg/mL.
结论:
- 优化的IM-MS方法提供了芬太尼异构体的可靠检测和量化.
- 使用金属添加物和先进的数据处理提高了芬太尼识别的分析准确性.
- 这种方法为法医和临床实验室在解决阿片类药物危机方面提供了宝贵的工具.
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