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CD73 失调多发性骨髓瘤中单细胞抗瘤活性

Lin Zhou¹, XiaoLan Liu², Tao Guan²

¹Department of Hematology, The First Hospital of Shanxi Medical University, Taiyuan, Shanxi, People's Republic of China.

Cancer management and research

|July 26, 2023

概括

在多发性骨髓瘤中,CD73有助于免疫抑制瘤微环境. 抑制CD73部分恢复抗瘤免疫细胞功能,提供一种潜在的治疗策略.

科学领域:

免疫学免疫学免疫学
在瘤学瘤学.
生物化学生物化学

背景情况:

多发性骨髓瘤 (MM) 的特征是瘤微环境 (TME) 内免疫细胞功能障碍.
CD73是一种酶,在各种癌症中与瘤进展和免疫逃避有关.
CD73在MM TME中调节抗瘤免疫力的作用尚未完全阐明.

研究的目的:

研究MM TME内的免疫细胞中CD73的表达和功能.
评估CD73抑制对MM的抗瘤免疫反应的影响.

主要方法:

流细胞计被用来分析CD73表达T-,B-,NK-淋巴细胞和单细胞从骨髓 (BM) 和周围血液 (PB) 的MM患者和健康对照.
用RPMI-8226细胞和CD73抑制剂进行共同培养实验,以评估抗瘤活性.
细胞计数珠阵列被用来测量细胞因子水平 (IL-2,IL-4,IL-6,IL-10,TNF-α,IFN-γ).
观察和量化了单细胞细胞症.

主要成果:

与健康对照人群相比,MM患者的淋巴细胞和单细胞中CD73的表达高.
在MM单细胞中,免疫抑制性细胞因子TNF-α,IFN-γ和IL-10的水平升高.
在MM免疫细胞中抑制CD73显著增强了IL-2,TNF-α和IFN-γ的分泌,改善了免疫细胞杀死能力.
在MM单细胞中抑制CD73特别促进了IL-2,TNF-α和IFN-γ的分泌,增强了杀死和细胞活性.

结论:

CD73通过破坏单细胞抗瘤效应,有助于多发性骨髓瘤中免疫抑制的TME.
向CD73是恢复MM患者抗瘤免疫力的有希望的治疗策略.

关键词:

CD73 CD73 CD73 CD73 CD73 CD73 CD73 CD73 CD73 CD73 CD73 它具有抗瘤活性.免疫细胞免疫细胞单细胞 (Monocytes) 是一种单细胞.多发性骨髓瘤是多发性骨髓瘤的一种.发酵细胞的形成发酵细胞的形成

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