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精简和按需制备mRNA产品在一个通用集成平台上

Hongjuan Wei¹, Zhen Rong¹, Liyan Liu¹

¹Bioinformatics Center of AMMS, Beijing, 100850 P. R. China.

Microsystems & nanoengineering

|July 26, 2023

概括

一个新的集成平台简化了信使RNA (mRNA) 生产,以快速开发疫苗和个性化疗法. 这项技术可以根据需求准备mRNA产品,加速对COVID-19等疾病的反应.

科学领域:

生物技术是生物技术.
分子生物学分子生物学
疫苗学疫苗学疫苗学

背景情况:

与传统疫苗相比,信使RNA (mRNA) 疫苗提供了快速开发和成本有效的生产.
现有的mRNA疫苗开发可能是耗时和资源密集的,阻碍了对新出现的疾病的快速反应.
需要简化,按需的mRNA生产平台,以应对急性健康威胁和个性化医疗.

研究的目的:

建立一个通用的综合平台,以简化和按需准备直接从DNA模板中获取mRNA产品.
以增强的绿色光蛋白 (eGFP) 为模型来证明平台的效率和能力.
生产一种针对SARS-CoV-2受体结合域 (RBD) 的mRNA候选疫苗.

主要方法:

使用聚合酶链反应 (PCR) 的目标DNA模板的体外放大.
在体外转录DNA模板成mRNA序列.
磁性净化mRNA和封装在脂质纳米颗粒.

主要成果:

该平台成功产生了增强绿色光蛋白 (eGFP) mRNA,该mRNA在HEK-293T细胞中得到了验证.
估计8小时内生产200-300微克eGFPmRNA的批量生产速度.
成功生产了一种编码SARS-CoV-2尖端蛋白的受体结合域 (RBD) 的mRNA疫苗.

结论:

开发的集成平台可实现通用和按需的mRNA产品制备.
这项技术促进了对mRNA技术的更广泛使用,以便在流行病期间快速开发疫苗.
该平台具有针对瘤学和慢性传染病的基于mRNA的个性化治疗的潜力.

关键词:

材料科学是一种材料科学.纳米颗粒纳米颗粒

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