毛皮蛋白质是导致番茄叶的原因 新德里番茄中的病毒病原性
Thuy T B Vo1, Aamir Lal2, Bupi Nattanong1
1Department of Integrative Biotechnology, Sungkyunkwan University, Suwon, Republic of Korea.
Frontiers in plant science
|July 26, 2023
概括
番茄叶卷新德里病毒 (ToLCNDV) 在番茄中的感染性是由其DNA-A段和外套蛋白 (CP) 决定的. 在CP的位置143的一个特定的氨基酸对ToLCNDV的致病性至关重要.
科学领域:
- 植物病毒学 植物病毒学
- 分子植物病理学 分子植物病理学
- 农作物科学 农作物科学
背景情况:
- 番茄叶曲线新德里病毒 (ToLCNDV) 是一种双重的贝戈莫病毒,在全球范围内造成了大量的作物损失.
- 亚洲 (ToLCNDV-In) 和地中海 (ToLCNDV-ES) 隔离物在番茄品种中表现出不同的致病性.
研究的目的:
- 在番茄植物中比较ToLCNDV-In和ToLCNDV-ES的致病性.
- 为了确定病毒遗传决定因素负责的ToLCNDV感染性和病原性在西红.
- 阐明ToLCNDV外套蛋白 (CP) 与宿主因子的分子相互作用.
主要方法:
- 在三种番茄品种中进行基于克隆的传染病原性测定.
- 在ToLCNDV-In和ToLCNDV-ES之间进行基因组交换实验.
- 构建和分析模拟的ToLCNDVDNA-A片段.
- 酵母两杂交查以识别CP相互作用蛋白.
- 定量逆转录PCR (RT-qPCR) 用于分析基因表达.
主要成果:
- ToLCNDV-In感染了所有测试的番茄品种,而ToLCNDV-ES没有.
- ToLCNDV DNA-A 分段被确定为西红感染性的主要决定因素.
- 发现ToLCNDV的外衣蛋白 (CP) 对病原性至关重要.
- 在CP中的氨基酸位置143对于ToLCNDV感染性至关重要.
- 观察到ToLCNDV CP和西红环指蛋白44-like之间的差异性相互作用.
结论:
- 在番茄中,ToLCNDV的DNA-A段,特别是外衣蛋白 (CP) 是其感染性和病原性的关键决定因素.
- CP的143氨基酸在ToLCNDV宿主相互作用和感染中起着至关重要的作用.
- 病毒性CP与宿主蛋白的明显相互作用,如环指蛋白44-like,可能是ToLCNDV分离物之间差异性致病性的基础.
相关概念视频
02:22Introduction to Plant Diversity
45.0K
From Water to Land
45.0K
01:29Subviral Agents
42
Subviral agents are infectious entities that resemble viruses but lack one or more viral components, such as a capsid or essential replication machinery. These agents include viroids, prions, and satellites, each possessing distinct structural and functional characteristics that influence their mode of infection and replication.Viroids are the simplest subviral agents, consisting of circular, single-stranded RNA molecules without a protein coat. They exclusively infect plants, relying entirely...
42
00:56Viral Structure
62.5K
Viruses are extraordinarily diverse in shape and size, but they all have several structural features in common. All viruses have a core that contains a DNA- or RNA-based genome. The core is surrounded by a protective coat of proteins called the capsid. The capsid is composed of subunits called capsomeres. The capsid and genome-containing core are together known as the nucleocapsid.
62.5K
02:28Leaky Scanning
5.2K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.2K