虫肠道上皮质不会变得更容易透光德克斯和细菌在寒冷中
Mahmoud I El-Saadi1, Kaylen Brzezinski1, Aaron Hinz1
1Department of Biology, Carleton University, Ottawa K1S 5B6, Canada.
The Journal of experimental biology
|July 26, 2023
概括
暴露于寒冷不会导致虫的肠道泄漏,这挑战了以前的假设. 这项研究质疑昆虫在寒冷压力下肠道透性的常见标记物的可靠性.
科学领域:
- 昆虫生理学 昆虫生理学
- 免疫学 免疫学 免疫学
- 环境压力反应环境压力反应
背景情况:
- 昆虫的肠道调节了离子和水的平衡.
- 众所周知,寒冷压力会导致昆虫肠道中的溶液泄漏.
- 寒冷压力可以激活昆虫的免疫反应,但肠道透性作为触发因素的作用尚不清楚.
研究的目的:
- 为了调查昆虫中冷感引起的肠道泄漏是否可以触发免疫反应.
- 在寒冷条件下评估昆虫肠道的透性.
- 为了评估光标记物对昆虫肠道透性的有效性.
主要方法:
- 虫 (Locusta migratoria) 用光细菌作为食物.
- 虫被暴露在-2°C的温度下,长达48小时.
- 活体肠囊制剂被用于测量FITC-dextran泄漏.
主要成果:
- 在暴露于寒冷的虫的血淋巴中没有检测到光细菌.
- 活体实验表明,在对寒冷的反应中,FITC-dextran通过肠道的泄漏没有增加.
- 这些发现质疑FITC-dextran用于评估冷诱导肠道透性的使用.
结论:
- 暴露在寒冷中似乎不会导致虫显著的肠道泄漏.
- 这项研究对FITC-dextran作为昆虫肠道透性的标记物的可靠性提出了疑问.
- 需要进一步的研究来了解在寒冷压力下昆虫肠道的功能.
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