circSLC25A13作为一个ceRNA,通过miR-616-3p/ADCY2轴调节AML的进展
Wenwen Wei1,2,3,4, Jiajia Pan1,2,3,4, Jinghan Wang1,2,3,4
1Department of Hematology, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, People's Republic of China.
Molecular carcinogenesis
|July 26, 2023
概括
一种新型的循环RNA,circSLC25A13,在急性髓性白血病 (AML) 中升高,并促进癌症的进展. 这一发现表明circSLC25A13是AML治疗的潜在生物标志物和治疗标.
科学领域:
- 分子生物学分子生物学
- 在瘤学瘤学.
- 在RNA生物学,RNA生物学.
背景情况:
- 循环RNAs (circRNAs) 是白血病的关键调节者.
- circRNAs正在成为癌症的显著预后因素.
- 了解急性髓性白血病 (AML) 中的新型circRNAs至关重要.
研究的目的:
- 在AML.中识别和鉴定一种新型circRNA,circSLC25A13的特征.
- 调查circSLC25A13在AML进展和存活中的作用.
- 阐明circSLC25A13在AML中的功能背后的分子机制.
主要方法:
- 在AML患者中分析circSLC25A13的表达.
- 在AML细胞中对circSLC25A13进行了体外和体内淘汰试验.
- 微RNA (miRNA) 海绵活动测定以确定circSLC25A13与miR-616-3p的相互作用.
- 下游目标基因表达的分析 (ADCY2).
主要成果:
- 在总生存率较低的AML患者中,circSLC25A13的表达显著更高.
- Knockdown 的 circSLC25A13 抑制 AML 细胞增殖,并诱导细胞亡.
- circSLC25A13作为miR-616-3p的miRNA海绵,导致增强的腺酸环酶2 (ADCY2) 表达.
- 通过ADCY2的上调和miR-616-3p的下调,可以部分挽救circSLC25A13缺乏的效应.
结论:
- circSLC25A13促进AML的进展通过海绵miR-616-3p和调节ADCY2.2.
- circSLC25A13是AML的潜在预后生物标志物.
- circSLC25A13代表了AML治疗的一个有前途的治疗标.
相关概念视频
Abnormal Proliferation
4.6K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.6K
GPCRs Regulate Adenylyl Cylase Activity
5.7K
Some GPCRs transmit signals through adenylyl cyclase (AC), a transmembrane enzyme. AC helps synthesize second messenger cyclic adenosine monophosphate (cAMP). AC catalyzes cyclization reaction and converts ATP to cAMP by releasing a pyrophosphate. The pyrophosphate is further hydrolyzed to phosphate by the enzyme pyrophosphatase, which drives cAMP synthesis to completion. However, cAMP is rapidly degraded to 5′ AMP by the enzymes phosphodiesterase (PDE), preventing overstimulation of...
5.7K
Anaphase Promoting Complex
2.9K
The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
2.9K
Regulation of Nuclear Protein Sorting
2.4K
Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
2.4K
MicroRNAs
3.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K
Interactions Between Signaling Pathways
6.3K
Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
6.3K


