通过拉曼光谱对切除边缘的术内评估,以指导口腔癌手术
Yassine Aaboubout1,2, Maria R Nunes Soares1,3, Tom C Bakker Schut3,4
1Department of Pathology, Erasmus MC Cancer Institute, University Medical Center Rotterdam, PO Box 2040, 3000 CA Rotterdam, The Netherlands. g.puppels@erasmusmc.nl.
The Analyst
|July 26, 2023
概括
对口腔癌切除边缘的准确的手术内评估至关重要. 一种新的拉曼光谱技术提供了一种快速,客观的方法来测量边缘,改善了口腔状细胞癌患者的手术结果.
科学领域:
- 在瘤学瘤学.
- 频谱学是一种光谱学.
- 手术病理学手术病理学
背景情况:
- 口腔癌手术的目标是>5毫米的无瘤边缘,仅在15-26%的病例中实现.
- 瘤切除边缘的手术内评估 (IOARM) 可以改善手术结果,但目前的方法是不切实际的.
- 现有IOARM的局限性阻碍了其广泛的临床采用和患者受益.
研究的目的:
- 为IOARM开发和验证一种新的高波数拉曼光谱技术.
- 为了能够快速,客观,准确地在新鲜的口腔癌样本上进行切除边缘的手术内测量.
- 在口腔癌手术期间提高瘤切除的精度.
主要方法:
- 开发一个高波数拉曼光谱系统,使用薄光纤针探针.
- 创建一个组织分类模型,以区分口腔状细胞癌 (OCSCC) 和健康的口腔组织.
- 基于拉曼光谱数据的边缘长度预测模型的实施,并与他的病理学进行比较.
主要成果:
- 组织分类模型在OCSCC检测方面实现了0.85的灵敏度和0.92的特异性.
- 边缘长度预测模型显示,与基因病理学相比,平均差异为-0.17毫米.
- 拉曼光谱技术提供了快速和客观的边际评估.
结论:
- 高波数拉曼光谱是一种有前途的技术,用于口腔癌的客观IOARM.
- 这种技术可以显著提高瘤切除边缘的准确性,潜在地减少正边缘.
- 开发的系统提供了一个切实可行的解决方案,以克服当前IOARM方法的局限性,使患者的护理受益.
相关概念视频
01:20Raman Spectroscopy: Overview
457
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
457
01:26Raman Spectroscopy Instrumentation: Overview
451
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
451