相关实验视频
Updated: Jul 21, 2025

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice
Published on: August 23, 2019
异常表达的 lncRNA LINC00847 可能成为甲状腺癌的有希望的预后因素
1Radiation Oncology, The Affiliated Hospital of Qingdao University, Qingdao University, Qingdao, China.
长非编码RNA LINC00847通过抑制细胞增殖和迁移,在甲状腺癌中起到瘤抑制作用. 它针对microRNAmiR-146b-5p,表明它有可能成为改善甲状腺癌治疗的预后生物标志物.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 甲状腺癌是由甲状腺毛囊上皮细胞引起的.
- 了解分子机制对于有效的治疗和预后至关重要.
- 识别新的预后生物标志物是一个关键的研究领域.
研究的目的:
- 研究LINC00847和miR-146b-5p在甲状腺癌中的作用.
- 探索它们作为分子预后生物标记物的潜力.
- 为了阐明LINC00847和miR-146b-5p在甲状腺癌进展中的调节关系.
主要方法:
- 实时定量聚合酶连锁反应 (RT-qPCR) 用于基因表达分析.
- 细胞计数工具-8 (CCK-8) 和Transwell对细胞增殖,迁移和入侵进行测试.
- 生物信息学工具和 luciferase 记者测试以确认海绵活动.
- 卡普兰-梅尔和考克斯回归分析用于预后价值评估.
主要成果:
- 在甲状腺癌组织和细胞中,LINC00847的表达减少,而miR-146b-5p在甲状腺癌组织和细胞中升级.
- 过度表达LINC00847显著抑制了甲状腺癌细胞的增殖,迁移和入侵.
- LINC00847直接准和抑制miR-146b-5p,对其表达进行负面调节.
- 高LINC00847表达与良好的预后有关.
结论:
- LINC00847 作为甲状腺癌中的瘤抑制剂.
- 该LINC00847/miR-146b-5p轴在调节甲状腺癌进展方面发挥着至关重要的作用.
- 作为甲状腺癌的有价值的预后标志物,LINC00847具有前景.
更多相关视频
03:37Author Spotlight: Impact of Intergenic Interactions on Disease-Identifying Dark Biomarkers
Published on: March 1, 2024
06:08Establishment and Characterization of Patient-Derived Xenograft Models of Anaplastic Thyroid Carcinoma and Head and Neck Squamous Cell Carcinoma
Published on: June 2, 2023
相关概念视频
lncRNA - Long Non-coding RNAs
Non-LTR Retrotransposons
Loss of Tumor Suppressor Gene Functions
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
Abnormal Proliferation
The Nucleolus
Cancer-Critical Genes II: Tumor Suppressor Genes
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...