来自Hevea brasiliensis的乳C血清诱导了白血病细胞系中的细胞死亡
Hansuk Buncherd1, Sawitree Hongmanee1, Charinrat Saechan1
1Faculty of Medical Technology, Prince of Songkla University, 15, Kanjanavanit Rd. Hat Yai, Songkhla, 90110, Thailand.
Molecular biology reports
|July 26, 2023
概括
希维亚巴西林氏乳C血清通过触发亡,有效地抑制白血病细胞的生长. 这种天然化合物激活关键的酶,为白血病提供潜在的治疗途径.
科学领域:
- 生物化学 生化学
- 细胞生物学 细胞生物学
- 免疫学 免疫学 免疫学
背景情况:
- 河巴西乳主要用于生产.
- 之前的研究表明,C血清对肝细胞癌的抗增殖作用通过非亡途径.
- 在白血病中,乳C血清的抗增殖机制仍然未被描述.
研究的目的:
- 为了研究Hevea brasiliensis乳C血清对人类白血病细胞系的抗增殖作用.
- 阐明潜在的分子机制,特别关注亡诱导和酶激活.
主要方法:
- 在K562,U937白血病细胞系和人体外围血液单核细胞 (PBMCs) 中使用MTT测定来评估细胞活力.
- 通过流细胞计 (附录素V/PI染色) 检测到亡.
- 使用qRT-PCR和酶分析分别量化了亡标记物 (BAD,BAX,CASPASE3) 和caspase活动 (caspase-8, -9, -3) 的基因表达.
主要成果:
- 乳C血清在K562和U937细胞中显著抑制了增殖,对PBMCs没有影响.
- 在治疗后的白血病细胞系中观察到早期和晚期亡细胞的显著增加.
- 在接受治疗的白血病细胞中,证实了亲亡基因 (BAD,BAX,CASPASE3) 的升调和caspase-8, -9和-3的激活.
结论:
- 赫维亚巴西林西斯乳C血清表现出强大的抗白血病活性.
- 抗扩散效应通过诱导亡和激活内在和外在的caspase通路来调节.
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