Search research articles

关于 JoVE

概览领导团队博客 JoVE 帮助中心

作者

出版流程编辑委员会范围与政策同行评审常见问题投稿

图书馆员

用户评价订阅访问资源图书馆顾问委员会常见问题

研究

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments 存档

教育

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual 教师资源中心教师网站

使用条款与条件

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序

Same author

The tetraspanin disc proteins, peripherin-2 and ROM1, facilitate CNG channel localization to the rod outer segment.

Molecular biology of the cell·2026

Same author

Dysregulation of Non-Muscle Myosin IIA Assembly and Phosphorylation in S100A4 Null Mouse Lens.

Journal of cellular physiology·2026

Same author

The Human Lens Fiber Cytoskeletome Reveals Neuronal Signatures and the Presence of Chaperonins, Proteasome, Signaling, and Redox Regulators.

Investigative ophthalmology & visual science·2026

Same author

Absolute quantification of fluorescent protein fusions by mass spectrometry.

Protein science : a publication of the Protein Society·2026

Same author

Adam9-deficient retinal pigment epithelium pseudopods maintain photoreceptor outer segment renewal despite subretinal space expansion.

The Journal of clinical investigation·2026

Same author

Publisher Correction: Multiple oestradiol functions inhibit ferroptosis and acute kidney injury.

Nature·2026

Search research articles

相关实验视频

Updated: Jul 21, 2025

Two Peeling Methods for the Isolation of Photoreceptor Cell Compartments in the Mouse Retina for Protein Analysis

Two Peeling Methods for the Isolation of Photoreceptor Cell Compartments in the Mouse Retina for Protein Analysis

Published on: December 7, 2021

摄影受体外段蛋白质的绝对量化

Nikolai P Skiba¹, Tylor R Lewis¹, William J Spencer¹

¹Department of Ophthalmology, Duke University School of Medicine, Durham, North Carolina 27710, United States.

Journal of proteome research

|July 26, 2023

概括

研究人员精确量化了老鼠棒外段中的20种蛋白质,解决了丰富数据中的差异. 这有助于我们更好地理解光传导途径.

关键词:

绝对的蛋白质量定量.一个光感受器.光传导是指光传导的方法.蛋白质组学蛋白质组学视网膜视网膜视网膜是一个视觉视觉视觉是一个视觉.

更多相关视频

Author Spotlight: Improved Lipofuscin Models and Quantification of Outer Segment Phagocytosis Capacity in Highly Polarized Human Retinal Pigment Epithelial Cultures

Author Spotlight: Improved Lipofuscin Models and Quantification of Outer Segment Phagocytosis Capacity in Highly Polarized Human Retinal Pigment Epithelial Cultures

Published on: April 14, 2023

Large-Scale Purification of Porcine or Bovine Photoreceptor Outer Segments for Phagocytosis Assays on Retinal Pigment Epithelial Cells

Large-Scale Purification of Porcine or Bovine Photoreceptor Outer Segments for Phagocytosis Assays on Retinal Pigment Epithelial Cells

Published on: December 12, 2014

相关实验视频

Last Updated: Jul 21, 2025

Two Peeling Methods for the Isolation of Photoreceptor Cell Compartments in the Mouse Retina for Protein Analysis

Two Peeling Methods for the Isolation of Photoreceptor Cell Compartments in the Mouse Retina for Protein Analysis

Published on: December 7, 2021

Author Spotlight: Improved Lipofuscin Models and Quantification of Outer Segment Phagocytosis Capacity in Highly Polarized Human Retinal Pigment Epithelial Cultures

Author Spotlight: Improved Lipofuscin Models and Quantification of Outer Segment Phagocytosis Capacity in Highly Polarized Human Retinal Pigment Epithelial Cultures

Published on: April 14, 2023

Large-Scale Purification of Porcine or Bovine Photoreceptor Outer Segments for Phagocytosis Assays on Retinal Pigment Epithelial Cells

Large-Scale Purification of Porcine or Bovine Photoreceptor Outer Segments for Phagocytosis Assays on Retinal Pigment Epithelial Cells

Published on: December 12, 2014

科学领域:

分子生物学分子生物学
细胞生物学细胞生物学
生物化学生物化学

背景情况:

眼睛中的光受体细胞捕获光线以启动视觉.
光传导,即将光转化为神经元信号的过程,发生在外侧部分.
之前的研究报告了相互矛盾的蛋白质丰度数据,阻碍了清晰的分子理解.

研究的目的:

准确量化20个关键蛋白质在老鼠棒外段的丰度.
解决报告中的蛋白质含量差异,并确定精确的摩尔比.
提高对光传导机械中的分子组织的理解.

主要方法:

使用定量质谱法同时测量蛋白质丰度.
确定了20种蛋白质的绝对分子数和分子比.
作为生物样本,使用了老鼠棒的外部段.

主要成果:

确定了20种结构和功能蛋白质的精确分子计数.
计算了蛋白质之间的摩尔比率,揭示了高准确度.
蛋白质复合体的量化显示,与已知的固态度的偏差不到7%.

结论:

这项研究提供了关于棒外段蛋白质丰度的最终数据集.
已解决的差异有助于我们更好地理解光传导中的分子组织.
这些发现支持将光传导途径视为协调的分子组合的观点.