有或没有功能化的氧化铁纳米颗粒通过增强器活动的表观遗传调节引起转录配置的变化
Federica Gamberoni1, Marina Borgese2, Christina Pagiatakis1,3
1Department of Biotechnology and Life Sciences, University of Insubria, via J.H. Dunant 3, 21100 Varese, Italy.
Nano letters
|July 26, 2023
概括
表观遗传变化,特别是像H3K27ac这样的基因组修饰,是了解纳米颗粒如何引起细胞损伤的关键. 这项研究将纳米粒子暴露与通过表观遗传修饰改变的基因表达联系起来.
科学领域:
- 纳米毒理学研究
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 分子生物学分子生物学
背景情况:
- 纳米粒子 (NP) 毒性背后的细胞机制尚未完全理解.
- 表观遗传学在NP不良影响中介作用需要进一步研究.
研究的目的:
- 在暴露于基于铁的磁性NP后,研究全基因组的组素修饰变化 (H3K27ac,H3K4me1,H3K9me2,H3K27me3).
- 为了将这些表观遗传变化与NIH3T3细胞的转录组变化相关联.
- 阐明表观遗传景观改变在纳米毒性的作用.
主要方法:
- 暴露NIH3T3细胞的铁基磁纳米粒子 (Fe2O3和Fe2O3@Co NPs).
- 对基因组基因组修饰的全基因组分析 (H3K27ac,H3K4me1,H3K9me2,H3K27me3).
- 转录组测序以评估基因表达变化.
主要成果:
- 纳米粒子暴露导致了H3K27ac.基因组分布的显著变化.
- H3K27ac分布的变化与转录反应有很强的相关性,特别是影响增强剂活性.
- 该研究确定了基于表观遗传改变的纳米毒性相关的特定基因组区域.
结论:
- 表观遗传场景的变化,特别是H3K27ac修饰,是推动基因表达变化和纳米毒性的关键机制.
- 表观遗传分析提供了一种全面的方法来评估纳米粒子毒性.
- 这种基因组区域的数据集可以增强当前的毒理学测试.
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