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绵羊的单细胞转录学特征概念的延长和植入

Gong-Xue Jia¹, Wen-Ji Ma², Zhao-Bo Wu³

¹Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, China; University of Chinese Academy of Sciences, Beijing 100049, China; Qinghai Key Laboratory of Animal Ecological Genomics, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, China.

|July 26, 2023

概括

动物怀孕依赖于子宫内膜-子宫内膜通信. 单细胞测序揭示了概念细胞如何分化和与子宫内膜相互作用,这对于成功的怀孕识别和延长至关重要.

科学领域:

生殖生物学生殖生物学
基因组学就是基因组学.
发展生物学发展生物学

背景情况:

概念体和子宫内膜之间的双向通信对于反动物的怀孕建立至关重要.
在怀孕早期了解细胞和分子相互作用对于生殖成功至关重要.

研究的目的:

剖析绵羊概念体和子宫内膜在植入前和周边阶段的转录组学动态.
为了确定细胞类型和分子途径,涉及到概念延长和怀孕识别.

主要方法:

绵羊概念体和子宫内膜的单细胞RNA测序.
转录组分析以确定细胞类型和基因表达模式.
对配对的转录进行综合分析,调解概念体-子宫内膜通信.

主要成果:

球状胚胎细胞有五种细胞类型;长长的概念细胞分化为17种细胞类型,显示出显著的细胞命运规范.
从极地 trofhectoderm 到 trofhoblast 的过渡会增加干扰素-tau 的表达,可能会启动 conceptus 的延长.
确定了13种子宫内膜衍生细胞类型,阐明了对概念发育的分子反应.
多个配对的转录,包括IGF2-IGF1R和FGF19-FGFR1,被确定为概念体-子宫内膜对话的媒介.

结论:

单细胞转录组学为反动物早期怀孕发展提供了前所未有的洞察力.
这项研究阐明了关键的细胞分化事件和分子通信通路,这些通路对于概念体延长至关重要.
这些发现为未来关于反动物生殖生物学的功能研究提供了宝贵的资源.

关键词:

CP: 发育生物学概念的延伸概念的延伸这是子宫内膜的内分泌物.胚胎外的胚胎膜是如何形成的干扰子-tau 的关系.配体-受体相互作用血统的确定血统的确定在植入前期的发展.这是一种反动物.皮肤上的托菲克托多德.子宫的感受性子宫的感受性

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