基因组变化参与了Staphylococcus aureus中化诺耐药性的发展
Thuc Quyen Huynh1,2,3, Van Nhi Tran1,3, Van Chi Thai1,3
1School of Biotechnology, International University, Ho Chi Minh City, Vietnam.
PloS one
|July 26, 2023
概括
黄金葡萄球菌 (Staphylococcus aureus) 的化 (FQ) 耐药性是由于目标突变和增加的排泄活动而出现的. 这项研究研究了在FQ暴露下多药耐药性 (MDR) 黄金色杆菌发展背后的机制.
科学领域:
- 微生物学 微生物学
- 遗传学 是一个遗传学.
- 药理学 药理学是指药理学的学科.
背景情况:
- 诺基诺 (FQ) 是关键的抗生素,但它们的有效性受到迅速出现的耐药性的威胁.
- 黄金葡萄球菌 (S. aureus) 可能发展出多药性耐药性 (MDR),这对临床构成重大挑战.
研究的目的:
- 为了研究基因组和分子机制驱动的MDR S. aureus菌株的出现暴露于诺基诺后.
- 为了确定特定的突变和基因表达变化与FQ耐药性相关的S. aureus.
主要方法:
- 序列次最小抑制度 (sub-MIC) 暴露S. aureusATCC 29213对西普罗夫洛克萨,奥洛克萨或莱沃弗洛克萨.
- 全基因组测序 (WGS) 和目标测序用于识别基因组变化.
- 用RT-qPCR量化涉及FQ耐药性,排泄和替代性西格玛因子的基因表达水平.
主要成果:
- 在暴露的S. aureus菌株中观察到FQ的最小抑制度 (MIC) 的显著和不可逆转的增加.
- 在FQ暴露的菌株中,WGS发现了多种突变,包括GrlA (R570H),SACOL0573和rimI.的变化.
- 在FQ耐药菌株中检测到与排泄 (norA,norB,norC,mgrA) 和替代西格玛因子 (sigB,sigS) 相关的基因过度表达.
结论:
- 在FQ压力下MDR S. aureus的出现与FQ目标序列内的突变密切相关.
- 排泄系统和它们的调节器的升级在S. aureus.中诺耐药性的发展中起着至关重要的作用.
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