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在人类乳腺癌中循环RNA介导的基因调节:生物信息学分析

Giulia Fiscon^1,2, Alessio Funari³, Paola Paci^1,2

¹Department of Computer, Control and Management Engineering, Sapienza University of Rome, Roma, Italy.

|July 26, 2023

概括

循环RNAs (circRNAs) 通过竞争的内源RNA (ceRNA) 网络来调节基因表达. 这项研究确定了乳腺癌中的新型circRNA-miRNA-mRNA相互作用,突出显示hsa_circRNA_102908,miR-410-3p和ESM1.1.

科学领域:

分子生物学分子生物学
基因组学就是基因组学.
癌症研究癌症研究

背景情况:

循环RNAs (circRNAs) 是生物功能和疾病的关键调节者.
竞争的内源RNA (ceRNA) 网络提供了一层转录后调节.
RNA相互作用影响疾病的发展,包括癌症.

研究的目的:

在人类乳腺癌中构建一个circRNA-miRNA-mRNA相互作用网络.
在这个网络中确定关键的监管三重生.
研究特定circRNAs在乳腺癌进展中的作用.

主要方法:

开发CERNOMA网络,分别表达的miRNAs (DEMs) 和circRNAs (DECs) 和mRNAs (DEGs) 的双部分图.
基于目标预测和逆表达趋势的circRNA-miRNA和miRNA-mRNA相互作用的预测.
在网络中识别放松管制的三胞胎.

主要成果:

塞尔诺玛网络成功地绘制了乳腺癌中的circRNA-miRNA-mRNA相互作用.
确定了一个显著的放松管制的三重组:上调的hsa_circRNA_102908,下调的miR-410-3p和上调的ESM1.
确定的三重组涉及与BRCA1相关的RING域1,这表明它在癌症中的作用.

结论:

这项研究为乳腺癌研究建立了一个新的circRNA-miRNA-mRNA网络 (CERNOMA).
确定的三重组 (hsa_circRNA_102908/miR-410-3p/ESM1) 是乳腺癌的一个潜在的关键调节器.
进一步研究这种三重体可能会揭示新的治疗点,以防止瘤扩散和转移.

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