Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Asymmetric Lipid Bilayer01:35

Asymmetric Lipid Bilayer

7.3K
Biological membranes show uneven distribution of different types of lipids in the inner and outer layers, resulting in transverse asymmetric membranes. The treatment of the erythrocyte membrane with the enzyme phospholipase confirmed the asymmetric nature of the lipid bilayer. The enzyme hydrolyzes lipids into fatty acids and hydrophilic groups. The phospholipase acts only on the outer layer of the membrane, while the inner layer remains intact. The phospholipase treatment resulted in 80%...
7.3K
Detergent Purification of Membrane Proteins01:18

Detergent Purification of Membrane Proteins

5.2K
Detergents are used to purify the integral proteins of the membrane. The hydrophobic portion of the detergent can replace membrane phospholipids while solubilizing the membrane proteins. When detergent monomers reach a specific concentration in a solution called critical micelle concentration (CMC), they form micelles. Above CMC, the concentration of the detergent monomers remains in equilibrium with the micelle. The number of detergent monomers present in the CMC varies for each detergent, and...
5.2K
Assembly of the Lipid Bilayer in the ER01:28

Assembly of the Lipid Bilayer in the ER

3.2K
Biological membranes are more than just a barrier separating cell cytoplasm from the outside environment. They are highly dynamic and help maintain the integrity and physiological stability of the cells as well as membrane-bound organelles. Membranes also play vital roles in cell-to-cell and intracellular communication.
A large chunk of any biological membrane is composed of phospholipids. These lipids have a heterogeneous distribution across different subcellular organelles and even between...
3.2K
Phosphoinositides and PIPs01:42

Phosphoinositides and PIPs

8.6K
Phosphoinositides are a group of phospholipids containing a glycerol backbone with two fatty acid chains and a phosphate attached to a myoinositol sugar ring. The inositol head group extends into the cytoplasm, where it is modified by adding phosphate groups to form phosphatidylinositol phosphates or PIPs.
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...
8.6K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

EGFR-Targeted and MMP-Activated Membranolytic Peptides Kill Cancer Cells Specifically In Vitro and Reduce Tumor Growth In Vivo.

Molecular pharmaceutics·2026
Same author

Visualising reaction complexes in amine-based unloaded and CO<sub>2</sub>-loaded carbon capture solutions.

Nature communications·2026
Same author

Assembling Lipid Membrane Scaffolds on Microgel-Based Artificial Cells through Vesicle Fusion onto the Hydrogel Network.

ACS nano·2026
Same author

Investigating the Interfacial Structure of Potato Protein Microgels at the Air-Water Interface.

Langmuir : the ACS journal of surfaces and colloids·2025
Same author

Heat-Induced Structural Changes in Lactoferrin for Enhanced Mucoadhesion.

ACS applied bio materials·2025
Same author

Solution structure of Titan-relevant aqueous ammonia by neutron diffraction.

Communications chemistry·2025

相关实验视频

Updated: Jul 21, 2025

Automated Lipid Bilayer Membrane Formation Using a Polydimethylsiloxane Thin Film
08:23

Automated Lipid Bilayer Membrane Formation Using a Polydimethylsiloxane Thin Film

Published on: July 10, 2016

18.4K

作为脂质支架基质的PDMS.

James A Goodchild1, Danielle L Walsh1, Harrison Laurent1

  • 1Molecular and Nanoscale Physics Group, School of Physics and Astronomy, University of Leeds, Leeds LS2 9JT, United Kingdom.

Langmuir : the ACS journal of surfaces and colloids
|July 26, 2023
PubMed
概括
此摘要是机器生成的。

聚甲基 (PDMS) 是支持脂质双层 (SLBs) 的通用基质. 虽然SLBs在PDMS上形成和运作,但其表面粗性会阻碍脂质相分离,而疏水性恢复会导致降解.

更多相关视频

Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions
12:18

Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions

Published on: August 3, 2021

3.6K
Author Spotlight: Advancing Cell Membrane Biophysics - Exploring Interactions and Challenges Through Experimental and Computational Approaches
07:31

Author Spotlight: Advancing Cell Membrane Biophysics - Exploring Interactions and Challenges Through Experimental and Computational Approaches

Published on: September 1, 2023

2.3K

相关实验视频

Last Updated: Jul 21, 2025

Automated Lipid Bilayer Membrane Formation Using a Polydimethylsiloxane Thin Film
08:23

Automated Lipid Bilayer Membrane Formation Using a Polydimethylsiloxane Thin Film

Published on: July 10, 2016

18.4K
Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions
12:18

Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions

Published on: August 3, 2021

3.6K
Author Spotlight: Advancing Cell Membrane Biophysics - Exploring Interactions and Challenges Through Experimental and Computational Approaches
07:31

Author Spotlight: Advancing Cell Membrane Biophysics - Exploring Interactions and Challenges Through Experimental and Computational Approaches

Published on: September 1, 2023

2.3K

科学领域:

  • 生物物理学的生物物理.
  • 材料科学 材料科学 材料科学
  • 表面化学 表面化学

背景情况:

  • 聚甲基 (PDMS) 是一种广泛使用的,具有成本效益的聚合物,用于微流体和器官芯片应用.
  • 它的弹性和易于制造使其对细胞膜的生物物理研究具有吸引力.
  • 然而,PDMS作为支持脂质双层 (SLBs) 的基质的兼容性需要进一步研究.

研究的目的:

  • 调查PDMS作为基质形成和研究支持脂质双层 (SLBs) 的适用性.
  • 描述PDMS表面特性和等离子体处理对SLB形成,扩散和稳定性的影响.
  • 确定使用PDMS在膜生物物理实验中的局限性和关键参数.

主要方法:

  • 原子力显微镜 (AFM) 分析PDMS表面地形和粗度.
  • 光光学显微镜观察脂质双层的形成,扩散和相位过渡.
  • 接触角测量以量化表面的水友性和监测疏水性恢复.

主要成果:

  • 脂质双层在血治疗的PDMS上成功形成,表现出正常的扩散和相变.
  • 表面粗的PDMS显著限制了脂质相分离和横向的水力动力流.
  • 随着时间的推移,双叶降解发生,与PDMS疏水回收相关,稳定性的关键水接触角度为30°.

结论:

  • PDMS是SLB形成和基础研究的合适基质,但其固有的粗性限制了更复杂的现象,如脂相分离.
  • 像氧等离子体这样的表面处理可以提高水友性,但不能完全消除粗的问题.
  • 了解PDMS疏水回收及其对双层完整性的影响,对于可靠的实验设计至关重要.