LC-MS-MS-MS3用于血液样本中∆9-四化甘醇和代谢物的确定和定量
Paula Proença1, Helena M Teixeira1,2, Beatriz Martinho1
1Forensic Chemistry and Toxicology Laboratory, National Institute of Legal Medicine and Forensic Sciences, Polo das Ciências da Saúde (Polo III), Azinhaga de Santa Comba, Coimbra 3000-548, Portugal.
Journal of analytical toxicology
|July 26, 2023
概括
法医毒理学现在有一个更快,更敏感的方法来检测血液中的THC等大麻素. 这种LC-MS-MS-MS3技术使用最小的样本体积 (0.1毫升) 进行准确的量化.
科学领域:
- 法医毒理学 法医毒理学
- 分析化学 分析化学
- 药理学 药理学是指药理学的学科.
背景情况:
- 在法医毒理学中经常检测到大麻素.
- 需要有效的方法来确定低度,使用最小的样本量和制备.
- 现有的方法可能缺乏法医分析所需的灵敏度或吞吐量.
研究的目的:
- 开发和验证一种高度选择性和高通量液体染色学-并联质谱法 (LC-MS-MS-MS3) 方法.
- 在血液样本中量化∆9-四大麻 (THC),11-基-∆9-四大麻 (THC-OH) 和11-nor-9-carboxy-∆9-四大麻 (THC-COOH).
- 证明LC-MS3在生物矩阵中药物确定中的实用性.
主要方法:
- 0.1毫升血液样本的蛋白质沉 (PPT) 使用乙尼.
- 逆相色谱使用一个Acquity UPLC® HHS T3柱与渐变化.
- 通过使用MS3和MRM模式的SCIEX QTRAP® 6500+三重四极线性离子陷质谱仪进行分析.
主要成果:
- 该方法表明选择性和线性 (r2 > 0.995) 在0.5到100 ng/mL之间.
- 精度和精度变化系数为<15%.
- 平均恢复率在88.0%至117.2%之间,没有显著的干扰化合物,矩阵效应或转移.
结论:
- 经过验证的LC-MS-MS-MS3方法对于量化血液中的大麻素具有敏感性,效率和稳定性.
- 这种方法只需要0.1毫升的血液,简化了样本的准备.
- 这项研究验证了LC-MS3作为一种可行的技术,用于在生物样本中分析药物.
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