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在蝶翅膀模式的发展中,Wnt信号通路成员的空间和时间调节
Tirtha Das Banerjee1, Suriya Narayanan Murugesan1, Heidi Connahs1
1Department of Biological Sciences, National University of Singapore, Singapore - 117557.
Science advances
|July 26, 2023
概括
独特的Wnt信号通路调节了蝶的翅膀模式. 本研究确定了特定的Wnt通路成员及其在蝶眼球和带发育中的作用,揭示了它们的相互作用.
科学领域:
- 发展生物学 发展生物学
- 遗传学 遗传学 是一个
- 进化生物学 进化生物学
背景情况:
- Wnt信号对于动物的发育至关重要,包括模式形成.
- 在蝶翅膀颜色图案 (眼球和带) 中,Wnt特定途径的参与尚未完全理解.
- 了解Wnt信号提供了对复杂特征演变的见解.
研究的目的:
- 研究蝶翅膀图案发育中的正规和非正规Wnt信号的定位和功能.
- 为了识别特定的Wnt配体和的受体参与眼球和带形成在Bicyclus anynana*.
- 为了阐明蝶翅膀图案中Wnt信号的时空调节.
主要方法:
- 位于Armadillo (Arm) 的位置,这是Wnt信号的关键组成部分.
- 在Bicyclus anynana*中,对所有八个Wnt配体和四个色受体进行了转录表达分析.
- 使用CRISPR-Cas9基因编辑选择的Wnt配体和受体的功能分析.
主要成果:
- 清晰的Wnt信号通路对于蝶翅膀的眼球和带纹路都至关重要.
- 阿马迪罗本地化表明了正规Wnt信号活动的特定领域.
- 表达式分析揭示了参与模式发展的多个Wnt通路组件的存在.
- CRISPR-Cas9实验为某些Wnt配体和受体的作用提供了功能性证据.
结论:
- Wnt信号通路在蝶翅膀眼球和带的发展中发挥着独特和必不可少的作用.
- 这些路径可能会相互作用,以调节模式元素的空间域.
- 这项研究阐明了蝶翅膀颜色底层的分子机制,并为未来关于模式进化的研究提供了基础.
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