结晶性对酶定向和动力学对金属有机框架中的生物矿物化的影响
Zoe Armstrong1, Austin MacRae1, Mary Lenertz1
1Department of Chemistry and Biochemistry, North Dakota State University, Fargo, North Dakota 58102, United States.
ACS applied materials & interfaces
|July 26, 2023
概括
生物矿物化将酶封装在金属有机框架 (MOF) 中. 位点定向旋转标记电子磁共振 (SDSL-EPR) 揭示了酶动态与MOF晶体包装质量相关,并影响催化性能.
科学领域:
- 材料科学 材料科学 材料科学
- 生物化学 生物化学
- 频谱学是一种光谱学.
背景情况:
- 水相联合结晶使酶在金属有机框架 (MOF) 中进行封装,扩大其作为酶载体的使用.
- 酶@MOF生物复合物的低结晶度引发了对MOF晶体包装质量影响酶性能的担忧.
- 由于有限的数据库和背景干扰,在MOF支架中探测酶动态具有挑战性.
研究的目的:
- 解决MOFs中探测酶动力学和限制的挑战.
- 研究MOF晶体包装质量与酶性能之间的关系.
- 建立定位旋转标记电子偏磁共振 (SDSL-EPR) 作为评估MOF质量的方法.
主要方法:
- 囊括lyszyme,一个模型的大基质酶,进入八个新发现的水性MOF,通过水相联合结晶,具有不同的晶度.
- 位点定向旋转标签 (SDSL) 与电子磁共振 (EPR) 光谱相结合,以探测残留水平上的酶动态.
- 计算模拟来分析EPR数据并将酶流动性与MOF属性相关联.
主要成果:
- 已确立的酶流动性/动力学和MOF晶体特性 (包装质量和密度) 之间的相关性.
- 在MOF中发现了酶的暴露表面积与它们的催化性能之间的联系.
- 证明SDSL-EPR可以有效地探测酶动态,并作为MOF晶体包装质量的指标.
结论:
- SDSL-EPR是一种强大的技术,用于评估生物复合材料中的酶动态和MOF质量.
- MOF晶体包装质量显著影响封装酶的移动性和催化活性.
- 这种方法可用于在各种固体表面探测酶动力学,并指导酶固定平台的合理设计.
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