与DNA甲基化和基因表达的并行转移向老化老鼠脏的平均值
Sangkyun Jeong1,2, Sunwha Cho2, Seung Kyoung Yang2
1Medical Research Division, Korea Institute of Oriental Medicine (KIOM), Yuseong-gu, Daejeon 34054, South Korea.
Aging
|July 26, 2023
概括
与年龄相关的DNA甲基化漂移显示出双向变化,在老化哺乳动物中,低甲基化区域变得高甲基化,高甲基化区域变得低甲基化.
科学领域:
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 基因组学就是基因组学.
- 衰老研究研究 衰老研究
背景情况:
- 哺乳动物的衰老的特征是与年龄相关的DNA甲基化漂移 (AMD),涉及全球低甲基化和局部高甲基化.
- 了解驱动这种双向AMD的机制对于理解衰老过程至关重要.
研究的目的:
- 研究哺乳动物双向AMD背后的原理.
- 分析老鼠脏DNA中空间聚集的CpG二核酸的甲基化状态.
主要方法:
- 减少代表性双硫酸盐测序 (RRBS) 用于研究DNA甲基化模式.
- 分析包括根据密度对CpG进行分类,并根据初始甲基化水平对基因组区域进行分组.
主要成果:
- 随着年龄的增长,平均CpG甲基化水平的全球下降被观察到.
- 促剂CpG变得过甲基化,而基因体和基因间CpG变得低甲基化.
- 高甲基化发生在CpG丰富的区域,低甲基化发生在CpG贫穷的区域,甲基化水平预测了AMD模式.
结论:
- 与年龄相关的高甲基化和低甲基化事件看起来是随机的,与功能障碍的甲基化维持机制有关.
- 与甲基化变化相关的基因表达向平均值的转变表明,表观遗传漂移和衰老期间的转录调节之间存在潜在联系.
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