TRIM21促进瘤进展和癌症干在宫状状细胞癌的宫状细胞癌
Yanru Yang1, Saijie Zuo2, Wenqing Li1
1State Key Laboratory of Cancer Biology, Department of Pathology, Xijing Hospital and School of Basic Medicine, Fourth Military Medical University, Xi'an, China.
Pathology, research and practice
|July 26, 2023
概括
三倍基因蛋白21 (TRIM21) 促进宫状细胞癌的进展,是不良的预后指标. 了解TRIM21的作用为这种癌症提供了潜在的治疗点.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 癌症研究 癌症研究
背景情况:
- 三倍基因蛋白21 (TRIM21) 涉及瘤细胞增殖,转移和瘤标记物的无处不在.
- 越来越多的TRIM21表达水平被认为可以预测癌症的预后.
- 在宫平细胞癌 (CESC) 中TRIM21的特定作用仍然在很大程度上未被阐明.
研究的目的:
- 研究CESC组织中TRIM21的表达水平.
- 为了确定TRIM21表达和患者预后之间的相关性.
- 阐明TRIM21在CESC细胞增殖,迁移,入侵和干细胞中的功能作用.
主要方法:
- 免疫组织化学,西部Blot和定量PCR (q-PCR) 用于评估113个CESC患者样本中的TRIM21表达.
- 进行了细胞增殖,迁移和入侵分析 (CCK-8,伤口愈合,Transwell).
- 用球形分析和异种移植模型来评估TRIM21对瘤干和瘤发育的影响.
主要成果:
- 在CESC组织中,TRIM21的表达在mRNA和蛋白质水平上明显增加,相比之下,在癌前组织中,TRIM21的表达明显增加.
- 更高的TRIM21表达与更差的预后相关,并与个体预后因素相关.
- TRIM21过度表达增强了CESC细胞的增殖,迁移,入侵和干细胞,而敲击抑制了这些过程和瘤生长 in vivo.
结论:
- TRIM21作为宫状状细胞癌的不良预后预测剂.
- TRIM21在促进CESC进步中的作用表明其作为治疗点的潜力.
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