相关实验视频

Updated: Jul 21, 2025

Author Spotlight: High-Throughput In Vivo Leaf Inoculation for Accelerating Disease Resistance Screening in Poplar Hybrid Breeding

Author Spotlight: High-Throughput In Vivo Leaf Inoculation for Accelerating Disease Resistance Screening in Poplar Hybrid Breeding

Published on: September 20, 2024

miR159a调节了树对不同真菌和细菌的抵抗力

Xiaoqian Yang¹, Tiantian Fu¹, Ruen Yu¹

¹State Key Laboratory of Tree Genetics and Breeding, National Engineering Research Center of Tree Breeding and Ecological Restoration, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, The Tree and Ornamental Plant Breeding and Biotechnology Laboratory of National Forestry and Grassland Administration, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, 100083, China.

Plant physiology and biochemistry : PPB

|July 26, 2023

概括

在树中过度表达microRNA159 (miR159) 改变了抗病能力,增加了对细菌的敏感性,但增强了对真菌的防御能力. 这表明miR159在协调植物病原体相互作用方面发挥着复杂的作用.

科学领域:

植物病理学植物病理学
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

植物面临着来自各种病原体的持续威胁.
了解 (Populus) 疾病反应中的调节机制至关重要.
微RNA159 (miR159) 是一种受保护的植物调节剂,参与发育和压力.

研究的目的:

研究米R159在对真菌和细菌病原体的反应中的作用.
阐明木中miR159介导的抗病性背后的分子机制.

主要方法:

产生过度表达pto-miR159a (OX-159) 的转基因.
用Cytospora chrysosperma,Lonsdalea populi和Collettotrichum gloeosporioide进行病原体注射的测定. 这种测试可以使用
转录组分析和基因表达造型 (SA,JA/ET通路).

主要成果:

OX-159树对L. populi的易感性增加,但对C. chrysosperma的耐药性增加.
OX-159系对C. gloeosporioide表现出更高的敏感性,ROS积累减少.
转录组数据显示了与防御相关的基因和关键信号通路媒介体 (NPR1,ERF,MPK3/6) 的改变表达.

结论:

过度表达pto-miR159a会对树的抗病性产生对抗作用.
miR159影响了酸和莉酸/乙烯信号通路之间的交叉声.
这项研究提供了关于miR159在调解树与病原体相互作用和疾病结果中的作用的见解.

关键词:

科莱托里克 (Colletotrichum gloeosporioides) 是一种的植物.细胞胞 (Cytospora chrysosperma) 是一种精子.疾病耐药性疾病耐药性伦斯达莱亚人民群众这是一棵树.在 miR159a 中使用.

更多相关视频

Poplar Adventitious Roots Induced by Stem Canker Pathogens: An Experimental System for Studying Roots Biology and Light Response-Related Processes

Poplar Adventitious Roots Induced by Stem Canker Pathogens: An Experimental System for Studying Roots Biology and Light Response-Related Processes

Published on: October 11, 2024

Bacterial Leaf Infiltration Assay for Fine Characterization of Plant Defense Responses using the Arabidopsis thaliana-Pseudomonas syringae Pathosystem

Bacterial Leaf Infiltration Assay for Fine Characterization of Plant Defense Responses using the Arabidopsis thaliana-Pseudomonas syringae Pathosystem

Published on: October 1, 2015

相关概念视频

Gene Regulation in Microbial Communities: Quorum Sensing

Gene Regulation in Microbial Communities: Quorum Sensing

Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...