CCDC176稳定了微管双体1和9,以确保适当的精子运动
Chao Liu1, Qianchun Wang2, Lusheng Gu3
1Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou 510623, China; State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
Current biology : CB
|July 26, 2023
概括
含有176 (CCDC176) 的蛋白质卷轴-卷轴域对男性生育能力至关重要. 它的缺失会破坏精子中关键的鞭毛结构的稳定,损害精子的运动能力,导致不孕.
科学领域:
- 生殖生物学 生殖生物学
- 精子运动机制 精子运动机制
- 分子细胞生物学 分子细胞生物学
背景情况:
- 精子鞭毛中不对称的轴膜结构的分子基础仍然不太清楚.
- 精子的鞭毛运动性对于受精是必不可少的,但基本的结构成分及其调节尚未完全阐明.
研究的目的:
- 为了研究含有176 (CCDC176) 的卷轴-卷轴域在精子鞭毛结构和功能中的作用.
- 阐明CCDC176影响精子运动和男性生育能力的分子机制.
主要方法:
- 产生和分析Ccdc176淘汰赛小鼠.
- 使用免疫光和超分辨率成像进行精子分析.
- 评估精子运动性,鞭毛节拍模式和游泳路径.
- 应用精子多体动力学 (MBD) 模型.
主要成果:
- Ccdc176淘汰会导致男性不育,并显著减少精子的运动性.
- CCDC176是不对称地沿着精子轴膜局部化,并稳定微管双体 (MTDs) 1和9.
- 缺少CCDC176会导致MTDs1和9的不稳定,导致结构缺陷和 flagellar动态的改变.
- 在MTDs 1和9中的缺陷会影响精子的鞭毛节拍模式和游泳路径,影响受精潜力.
结论:
- CCDC176对于稳定精子鞭毛内特定的微管双体 (MTDs 1 和 9) 是必要的.
- CCDC176对MTDs 1和9进行适当的稳定对于正常的精子运动和男性生育能力至关重要.
- CCDC176代表了理解和治疗男性不孕症的潜在目标.
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