对牛SULT1家族的进化和功能特征的分析
Kosuke Sato1, Kiyoshi Yamauchi1, Akinori Ishihara1
1Department of Biological Science, Faculty of Science, Shizuoka University, Shizuoka 422-8529, Japan.
General and comparative endocrinology
|July 26, 2023
概括
研究人员确定了牛硫转移酶1 (SULT1) 家族,揭示了六个子家族. 特定的SULT1Cc1和SULT1Y1酶表现出高活性,并且可能参与甲状腺激素在转化过程中的清除.
科学领域:
- 进行比较的基因组学.
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 硫转移酶 (SULT) 是异生菌和激素代谢中的关键酶.
- SULT1家族在脊椎动物中存在,但其在两动物中的特定作用,特别是在变形过程中,尚未完全理解.
- 牛 (Rana catesbeiana) 在转型过程中经历了显著的生理变化,涉及复杂的荷尔蒙调节.
研究的目的:
- 为了识别和表征牛SULT1基因家族.
- 调查关键牛SULT1成员的酶活性和基质特异性.
- 探索牛SULT1酶在转化过程中的甲状腺激素代谢中的潜在作用.
主要方法:
- 使用BLAST对公共数据库进行搜索的生物信息分析.
- 牛SULT1家族成员的鉴定和遗传学分析.
- 对编码为SULT1Cc1和SULT1Y1.1的cDNA进行克隆.
- 使用2-纳夫托和3'-adenosine 5'-phosphosulfate (PAPS) 来表达重组蛋白和分析硫转移酶活性.
- 使用甲状腺激素类似物进行酶动力学和抑制试验.
主要成果:
- 雄牛青SULT1家族包括六个子家族,其中四个与哺乳动物SULT1相关,两个新型SULT1Cc成员.
- 克隆的公牛青SULT1Cc1和SULT1Y1酶表现出显著的硫转移酶活性.
- 与SULT1Y1.1.1相比,SULT1Cc1对2-naphthol和PAPS的亲和力更高,反应速度更快.
- 甲状腺激素类似物抑制SULT1Cc1和SULT1Y1对2-naphthol的硫化活性.
- 针对甲状腺素 (T4) 的SULT1Cc1和SULT1Y1的硫化功效表明它们参与了细胞内T4清除.
结论:
- 雄牛青SULT1家族是多样化的,有独特的两动物特有的子家族.
- 牛SULT1Cc1和SULT1Y1酶具有催化活性,在代谢异生菌和潜在的激素方面发挥作用.
- 这些酶很可能参与甲状腺激素在牛转型过程中的细胞内清除,从而有助于这种发育过程的荷尔蒙调节.
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