微管相关的隔膜复合体调节了基因素和基因素的运动性,具有不同的特异性
Yani Suber1, Md Noor A Alam1, Konstantinos Nakos1
1Department of Biology, Drexel University, Philadelphia, Pennsylvania, USA.
The Journal of biological chemistry
|July 26, 2023
概括
七复合体调节了微管沿着分子电机的运动,影响了膜流量. 不同的隔膜组合控制运动活动,影响细胞结构和功能.
科学领域:
- 细胞生物学 细胞生物学
- 分子电机分子电机
- 细胞骨动力学 细胞骨动力学
背景情况:
- 长距离的膜传输依赖于微管相关的蛋白质和翻译后的修改,形成一个"交通代码".
- 控制这种代码和运动蛋白 (素和素) 编排的监管原则在很大程度上是未知的.
- 七是GTP结合蛋白,形成复合体并与微管结合,可能影响运动活动.
研究的目的:
- 研究特定的隔膜复合物 (SEPT2/6/7,SEPT2/6/7/9,SEPT5/7/11) 如何在体外影响基因素 (KIF5C,KIF1A) 和二烯-二烯-二甲D (DDB) 复合物的运动性.
- 为了确定单个隔膜子单元是否为隔膜复合体贡献不同的调节性质.
- 为了检查膜交通和海马神经元内的戈尔吉组织中septins的体内作用.
主要方法:
- 单分子体外运动性测试用于观察隔膜涂层微管上运动蛋白的行为.
- 生物化学试验测试纯化隔膜复合体对素和素运动活性的影响.
- 使用海马神经元进行体内研究,以评估隔膜枯竭对戈尔吉形态和膜流量的影响.
主要成果:
- SEPT2/6/7通过阻止微管结合,强烈抑制DDB和KIF5C,并通过阻碍步进来抑制KIF1A.
- 在SEPT2/6/7/9复合体中的SEPT9抑制了KIF1A的抑制,表明了子单元特定的调节.
- SEPT5/7/11允许KIF1A运动,同时使DDB停滞不前,而神经元中的SEPT5耗尽会破坏Golgi形态和两极分化,这表明SEPT5/7/11.11将DDB连接起来.
结论:
- 微管相关的隔膜复合体在调节微管运动和定位方面表现出差异性的特异性.
- 各个隔膜子单元在确定隔膜复合体的调节功能方面发挥着至关重要的作用.
- 七是基于微管的代码的组成部分,该代码在空间上控制了膜流量.
相关概念视频
Microtubule Associated Motor Proteins
8.1K
Eukaryotic cells have different motor proteins for transporting various cargo within the cell. These motor proteins differ based on the filament they associate with, the direction they move within the cell, and the type of cargo they transport. Motor proteins that associate with microtubules are known as microtubule-associated motor proteins. There are two families of microtubule-associated motor proteins —Kinesins and Dyneins. Both these proteins assist in the transport of cellular...
8.1K
Role of Septins
1.8K
Septins are the recently discovered fourth major protein component of the cytoskeleton, along with microfilaments, microtubules, and intermediate filaments. These proteins can associate with other cytoskeletal filaments and carry out varied roles or can be free-floating in the cytoplasm.
Cellular Functions of Septins
Recent studies have revealed the multifaceted roles of septins in various cellular processes such as cytokinesis, ciliogenesis, and neurogenesis. Septins act as scaffolds and...
Cellular Functions of Septins
Recent studies have revealed the multifaceted roles of septins in various cellular processes such as cytokinesis, ciliogenesis, and neurogenesis. Septins act as scaffolds and...
1.8K
Microtubules in Cell Motility
3.3K
Microtubules are thick hollow cylindrical proteins that help form the cytoskeleton. Microtubules have varied roles in the cell. These filaments help form cellular appendages like cilia and flagella, which are responsible for locomotion. The cilia arise from basal bodies, separated from the main body by a membrane-like structure forming the transition zone. This zone is the gate for the entry of lipids and proteins, creating a unique composition of lipids and proteins in the ciliary membrane and...
3.3K
The Movement of Organelles and Vesicles
4.6K
In eukaryotic cells, cytoskeletal filaments such as actin, microtubules, and intermediate filaments form a mesh-like cytoskeletal network. These filaments serve as tracks for transporting cellular cargo. Specialized motor proteins use the chemical energy stored in adenosine triphosphate (ATP) for this transport. During interphase, microtubules are polarized, with the plus-end towards the cell periphery and the minus-end towards the cell center. Two microtubule-associated motor proteins,...
4.6K
Destabilization of Microtubules
2.8K
The destabilization of microtubules can occur during different stages of the microtubule lifecycle, such as nucleation or elongation. It can take place at either end of the microtubule or in the microtubule lattices as a whole. The lifespan of individual microtubules within a cell varies according to the cell type and stage of the cell cycle. During interphase, the lifespan of the microtubule is about 30 minutes, while during cell division, it is about 15 minutes. In axonal microtubules of...
2.8K
Assembly of Complex Microtubule Structures
1.9K
Complex microtubule structures are present in resting cells and in dividing cells. In resting cells, they are responsible for maintaining the cellular architecture, tracks for intracellular transport, positioning of organelles, assembly of cilia and flagella. They mediate the bipolar spindle assembly for chromosomal segregation and positioning of the cell division plate in dividing cells. The formation of microtubule complex structures depends on the cell type, cell stage, and cell function.
1.9K


