西诺因调节与circTRPM7相关的途径,以抑制胃癌细胞生长和转移
Jingwei Yan1, Jingqing Yang2, Huifen Shen1
1Department of Traditional Chinese Medicine, Yantai Affiliated Hospital of Binzhou Medical University, Yantai, China.
Chemical biology & drug design
|July 26, 2023
概括
在胃癌中,sinomenine通过调节circTRPM7/microRNA-145-5p/PBX3通路,抑制癌细胞生长和转移,表现出抗瘤特性.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 生物化学 生化学
背景情况:
- 西诺因在各种癌症中显示出抗瘤潜力,包括胃癌.
- 循环RNAs (circRNAs) 参与胃癌的进展,但它们与Sinomenine的相互作用尚不清楚.
研究的目的:
- 通过调节circRNA通路,调查Sinomenine是否通过调节circRNA通路在胃癌中发挥抗瘤作用.
- 阐明涉及circTRPM7,microRNA-145-5p和PBX3.3的特定分子机制.
主要方法:
- 定量实时PCR用于基因表达分析.
- 细胞测试 (MTT,殖民地形成,EdU,Transwell,伤口愈合,流动细胞计) 用于增殖,迁移,入侵和亡.
- 西方斑块用于蛋白质分析和分子相互作用的双露西法酶/RIP测定.
主要成果:
- 西诺因治疗减少了胃癌细胞中circTRPM7的表达.
- 过度表达circTRPM7促进了生长和转移,而circTRPM7则使miR-145-5p变为海绵,从而扭转了这些影响.
- miR-145-5p准了PBX3,PBX3的淘汰抵消了miR-145-5p的瘤抑制作用.
结论:
- 在胃癌中,sinomenine发挥了抗瘤作用.
- 这种效应通过circTRPM7/miR-145-5p/PBX3轴进行介导,突出显示了一种新的治疗途径.
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