相关实验视频
Updated: Jul 21, 2025
08:40
Gyroid Nickel Nanostructures from Diblock Copolymer Supramolecules
Published on: April 28, 2014
12.5K
通过扫描传输电子显微镜高分辨率的三维结构确定未染色的双状腺块共聚物
Ying Chen1, Jhih-Heng Yang1, Ya-Ting Chang2
1Department of Engineering and System Science, National Tsing Hua University, Hsinchu, 30013, Taiwan.
Scientific reports
|July 26, 2023
概括
我们将使用扫描传输电子显微镜 (STEM) 对未染色区块共聚物的第一个3D结构分析. 这项技术揭示了染色和未染色材料之间的纳米级结构差异.
科学领域:
- 材料科学 材料科学 材料科学
- 聚合物科学 聚合物科学
- 纳米技术纳米技术
背景情况:
- 块共聚合物材料为各种应用提供可调节的特性.
- 使用传输电子显微镜 (TEM) 的电子断层扫描是染色块共聚物的3D成像的标准.
- 扫描传输电子显微镜 (STEM) 与环状暗场 (ADF) 检测显示出分析未染色块共聚合物的前景.
研究的目的:
- 使用Cs纠正的STEM进行未染色区块共聚合物的3D结构分析.
- 调查STEM对光元素的分辨率限制和能力.
- 为了比较染色与未染色区块共聚合物标本的3D结构.
主要方法:
- 球形偏差Cs校正扫描传输电子显微镜 (STEM).
- 用于3D成像的环状暗场 (ADF) 检测.
- 200纳米厚的聚乙烯-聚乙烯 (PS-P2VP) 薄膜的3D重建.
主要成果:
- 实现了未染色的PS-P2VP块共聚合物的8.6nm分辨率的3D结构分析.
- 在z-对比重建中,从聚烯 (PS) 分子中分辨出聚-2-乙烯 (P2VP).
- 在染色和未染色的标本之间确定了不同的结构特征.
结论:
- 经过Cs校正的STEM能够对未染色的块共聚合物进行高分辨率的3D成像,克服了轻元素电子对比度的局限性.
- 这种方法通过揭示微妙的结构差异,为结构与属性关系提供了新的见解.
- 这项研究为分析不染色的复杂聚合物架构奠定了基础.
更多相关视频
09:37Optimized Negative Staining: a High-throughput Protocol for Examining Small and Asymmetric Protein Structure by Electron Microscopy
Published on: August 15, 2014
43.7K
09:22Self-assembling Morphologies Obtained from Helical Polycarbodiimide Copolymers and Their Triazole Derivatives
Published on: February 7, 2017
7.9K
相关概念视频
01:07Electron Microscope Tomography and Single-particle Reconstruction
2.4K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.4K
01:17Studying the Cytoskeleton
6.3K
The cytoskeletal architecture can be studied using different microscopic and biochemical techniques. Electron microscopy was instrumental in discovering the cytoskeletal architecture around the 1960s, which allowed obtaining structural information at a high-resolution level. However, the sample preparation procedure often limits this ability in biological samples. Several protocols have been developed over the years to optimize sample preparation. In one of the protocols known as rotary...
6.3K