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与神经毒性相关的miR-134-3p表达和zDHHC3-AMPARs轴的变化

Jing Song^1,2,3, Wenjing Li⁴, Chunman Yuan⁴

¹Department of Occupational Health, School of Public Health, Shanxi Medical University, Taiyuan, China. sj4933749@126.com.

Environmental science and pollution research international

|July 26, 2023

概括

职业暴露会增加miR-134-3p,这是一个与认知障碍相关的生物标志物. 这种微RNA向zDHHC3-AMPARs,影响学习和记忆,为神经毒性提供潜在的治疗标.

科学领域:

神经科学是一个神经科学.
毒理学毒理学毒理学
分子生物学分子生物学

背景情况:

(Al) 是一种已知的神经毒素,涉及认知功能障碍和神经退行性疾病,如阿尔茨海默氏症.
神经毒性背后的精确机制尚未完全理解.
微RNA-134-3p (miR-134-3p) 已经成为认知功能的调节者.

研究的目的:

研究miR-134-3p作为暴露个体认知功能障碍的潜在生物标志物.
阐明引发神经毒性的分子机制.
探索miR-134-3p/zDHHC3/AMPAR轴在引起的认知障碍中的作用.

主要方法:

在280名工厂工人的血中评估了miR-134-3p水平,与血液Al度和蒙特利尔认知评估 (MoCA) 分数相关.
利用动物模型和PC12细胞系验证诱导的miR-134-3p上调.
在PC12细胞中采用了转染和干扰技术,以调查zDHHC3的向及其对AMPAR贩运的影响.

主要成果:

职业暴露与血miR-134-3p水平升高和认知功能受损 (较低的MoCA分数) 相关.
在实验模型中,暴露上调了miR-134-3p表达.
发现miR-134-3p向棕化酶zDHHC3,导致AMPAR贩运减少,并导致学习和记忆缺陷.

结论:

miR-134-3p通过针对zDHHC3-AMPARs途径,与的神经毒性有关.
miR-134-3p可能作为暴露导致的认知障碍的潜在生物标志物.
针对miR-134-3p/zDHHC3-AMPARs轴是一个潜在的治疗策略,可以缓解引起的神经毒性.

关键词:

在AMPA受体上,AMPA受体是合金合金是一种认知障碍认知障碍是一种认知障碍.神经毒性神经毒性这种微型RNA-134-3p.zDHHC3C3 的时间.

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

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