从巴西UHT牛奶中分离出来的子形成细菌的遗传学特征和生物多样性
Isabella Maria Fernandes Botelho Moreira1, Rafaela da Silva Rodrigues1,2, Solimar Gonçalves Machado1
1InovaLeite - Laboratório de Pesquisa em Leites e Derivados, Departamento de Tecnologia de Alimentos, Universidade Federal de Viçosa, Viçosa, MG, 36570 900, Brazil.
概括
超高温 (UHT) 牛奶中的耐热子构成质量风险. 这项研究在巴西UHT牛奶中鉴定了子形成细菌,揭示了多种不同的细菌物种,主要是细菌细菌和细菌谷类菌群.
科学领域:
- 食品微生物学 食品微生物学
- 乳制品科学 乳制品科学
- 分子生物学分子生物学
背景情况:
- 来自污染微生物的耐热子对使用超高温 (UHT) 处理的乳制品公司来说是一个重大问题.
- 储存期间这些子的发芽可能会导致水解酶的产生,损害UHT牛奶的质量.
研究的目的:
- 为了调查来自巴西乳制品公司的UHT牛奶中的微生物污染.
- 识别和表征子形成细菌,特别是评估Bacillus sporothermodurans的存在.
- 为了确定UHT牛奶样本中的子形成细菌的生物多样性.
主要方法:
- 从184个UHT牛奶样本中分离和表型表征细菌.
- 生物化学试验用于初步鉴定,包括对Bacillus sporothermodurans的测试.
- 使用REP-PCR进行聚类和16SrDNA测序进行遗传学分析的分子鉴定.
主要成果:
- 没有一个分离物表现出Bacillus sporothermodurans的典型特征.
- 分子分析揭示了16个不同的分离集群.
- 基于16S rDNA测序的遗传学分析显示,分离物属于Bacillus subtilis和Bacillus cereus群体.
- 大多数分离物种之间的高系遗传关系使物种级别识别复杂化.
结论:
- 这项研究评估了来自评估地区的巴西UHT牛奶中分泌的污染物.
- 确定了UHT牛奶中子形成细菌的生物多样性,突出了Bacillus subtilis和Bacillus cereus群体的流行.
- 这些发现为进一步研究这些微生物对牛奶质量的影响开辟了道路.
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