在不同的酶基质比率下,Garcinia kola酶化水解物的生物活性
Salmat Adenike Salami1, Olukemi Adetutu Osukoya2, Olusola Bolaji Adewale1
1Biochemistry Programme, Department of Chemical Sciences, Afe Babalola University, Ado-Ekiti, Nigeria.
AMB Express
|July 26, 2023
概括
加尔西尼亚可拉种子的酶化解物显示出强大的抗氧化和抗炎作用. 以1:16的酶基质比率进行素水解,产生了最有效的生物活性,用于潜在的功能性食品和制药应用.
科学领域:
- 食品科学与技术 食品科学与技术
- 生物化学 生化学
- 药理学 药理学是指药理学的学科.
背景情况:
- 自然产品为合成化合物提供了安全的替代品.
- 加尔西尼亚可拉种子是一种丰富的生物活性化合物的来源.
- 酵素解剂和生物活性具有显著的健康益处.
研究的目的:
- 为了评估Garcinia kola pepsin酶化水解剂 (GKPHs) 的体外抗氧化和抗炎生物活性.
- 为了确定生产具有增强生物活性的GKPH的最佳酶基质比率.
主要方法:
- 通过性溶解和酸沉来分离加尔西尼亚可拉蛋白质.
- 在各种酶基质 (E:S) 比率下使用素对G. kola蛋白的酶性水解.
- 评估抗氧化活性 (DPPH,过氧化清理,铁离子化) 和抗炎活性 (膜稳定,抑制蛋白质变性).
主要成果:
- 在1:32的E:S比率下生产的GKPH表现出最高的水解度 (66.27%).
- 在所有测试比例中,GKPHs在体外表现出优异的抗炎性质.
- 1:16的E:S比率产生了具有最高DPPH清除活性 (87.24%),铁 (II) 化和蛋白质变性抑制 (53.36%) 的GKPH.
- 酶性化物也显示出出色的溶解性,乳化和发泡特性.
结论:
- 在1:16的E:S比率下,Garcinia kola蛋白的素水解产生具有卓越抗氧化和抗炎活性的酶性水解酸.
- 作为功能性食品和药品,GKPHs具有显著的发展潜力.
- 这些水解剂可以帮助管理与氧化应激和炎症相关的疾病.
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