用MXene感应的电化学发光传感器用于血栓活性检测和抑制剂查
Zijie Deng1,2, Xueping Tan1,2, Dongnan Guo1,2
1School of Pharmacy, Health Science Center, Xi'an Jiaotong University, 76 Yanta West Road, Xi'an, 710061, China.
Mikrochimica acta
|July 26, 2023
概括
使用Ti3C2Tx MXene的新型电化学发光 (ECL) 生物传感器有效检测血栓活性和屏幕抑制剂. 这种方法确定了强大的抑制剂,如异基,有助于血液凝固研究和药物发现.
科学领域:
- 生物化学 生物化学
- 材料科学 材料科学 材料科学
- 分析化学 分析化学
背景情况:
- 血栓激素在血液凝固和相关疾病中至关重要,需要精确的活性检测和抑制剂查,用于临床应用和药物开发.
- 现有的血栓检测和抑制剂查方法在灵敏度和效率方面存在局限性.
研究的目的:
- 开发一种敏感的电化学发光 (ECL) 生物传感器,用于检测血栓活性.
- 利用开发的生物传感器从自然来源选潜在的血栓抑制剂.
主要方法:
- 建立了一个ECL检测方法,使用Ti3C2Tx MXene作为Ru(bpy) 32+系统的敏感剂.
- 一种可以分离的血素基质 (bio-S-G-R-P-V-L-G-C) 用于评估血素活性.
- 生物传感器被用来选来自Artemisiae argyi Folium提取物的抑制剂.
主要成果:
- 该ECL生物传感器在0.1nU/mL至1μU/mL的线性范围内,在血清中检测血栓的检测极限为83pU/mL.
- 四种化合物 (异quercitrin,nepetin,L-camphor,L-borneol) 显示出50%以上的抑制,其中异quercitrin显示出90.26%的抑制.
- 鉴定出异基和尼佩丁是具有Ki值分别为0.91微米和2.18微米的竞争性抑制剂,得到了分子对接研究的支持.
结论:
- 开发的基于Ti3C2Tx MXene的ECL生物传感器提供了一个灵敏和高效的平台来检测血栓活性.
- 这种方法为选天然产品衍生的血栓激素抑制剂提供了宝贵的工具,可能导致新的治疗药物.
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