非编码RNA的识别和调节网络在病原体与宿主相互作用的微型菌的先天感染
Zigang Shen1,2,3, Qiong Yang4, Lie Luo1,3
1State Key Laboratory of Resource Insects, Southwest University, Tiansheng Street, Chongqing, 400715, People's Republic of China.
BMC genomics
|July 26, 2023
概括
这项研究确定了丝虫先天性微型菌病中的非编码RNA (ncRNA). 在N. bombycis感染期间,ncRNA网络对于病原体与宿主相互作用和丝虫发育至关重要.
科学领域:
- 基因组学就是基因组学.
- 分子生物学分子生物学
- 传染性疾病 传染性疾病
背景情况:
- 非编码RNA (ncRNA),包括长非编码RNA (lncRNA),圆形RNA (circRNA) 和微RNA (miRNA),对于生物过程和宿主-病原体相互作用至关重要.
- 由N. bombycis引起的丝虫的先天性微型菌病导致了重大经济损失,但ncRNAs在这种感染中的作用仍然基本不明.
研究的目的:
- 在先天感染期间,在N. bombycis和丝虫宿主中识别ncRNA及其调节网络.
- 阐明微型菌的先天性感染和宿主-病原体相互作用背后的分子机制.
主要方法:
- 在N. bombycis和丝虫胚胎/幼虫上进行了全转录组RNA测序 (RNA-Seq).
- 生物信息分析被用来识别和描述编码和非编码RNA.
- 竞争的内源RNA (ceRNA) 网络被构建以预测调控相互作用.
主要成果:
- 数以千计的mRNAs,lncRNAs,circRNAs和miRNAs在寄生虫和宿主中被确定.
- 构建了lncRNA/circRNA ceRNA网络,揭示了关键寄生虫基因 (例如,PTP3,SWP4) 和宿主基因 (例如,Toll-6,iNOS) 的调节.
- 跨物种分析强调了寄生虫和宿主miRNA在病原体与宿主相互作用中的重要作用.
结论:
- 这是第一次对丝虫中N. bombycis先天性感染进行全面的泛转录组研究.
- 在microsporidia先天性感染中,ncRNA介导的调节至关重要,为microsporidia生物学和宿主-病原体相互作用提供了新的见解.
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