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3D基因组结构变异在调节Brassica napus的种子油含量方面发挥着重要作用

Libin Zhang¹, Lin Liu², Huaixin Li¹

¹College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China; Key Laboratory of Molecular Biophysics of the Ministry of Education, Hubei Bioinformatics and Molecular Imaging Key Laboratory, Wuhan 430074, China.

Plant communications

|July 27, 2023

概括

基因组架构影响了Brassica napus中的种子油含量 (SOC). 定量特征位置 (QTL) 和相关基因组区域 (AGR) 的结构变化影响SOC调节,其中基因BnaA09g48250D发挥着关键作用.

科学领域:

植物遗传学植物遗传学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
基因组学就是基因组学.

背景情况:

在Brassica napus中,种子油含量 (SOC) 的调节是复杂的.
基因组架构在SOC中的作用尚不清楚.
了解SOC对于改善作物至关重要.

研究的目的:

研究3D基因组架构对Brassica napus中的SOC调节的影响.
识别涉及SOC变异的基因和基因组区域.
阐明 SOC 控制的基础分子机制.

主要方法:

高通量染色体构造捕获 (Hi-C) 用于分析3D基因组结构.
对差异可访问区域和基因表达的生物信息学分析.
多omics分析集成基因组学和转录组学.
对候选基因进行精细映射和功能验证.

主要成果:

高和低SOC Brassica napus线之间的3D基因组架构的显著差异.
在SOC定量特征位置 (QTLs) 和相关基因组区域 (AGRs) 内的不同表达的基因和可访问区域的丰富.
鉴定BnaA09g48250D作为调节SOC的关键基因,其表达与基因组结构变异有关.
功能验证证实了BnaA09g48250D在调节SOC中的作用.

结论:

在QTL/AGR中,3D基因组架构和结构变异显著影响Brassica napus中的SOC.
BnaA09g48250D是SOC的关键调节者,受基因组结构变异的影响.
这项研究从空间色素结构的角度对SOC调节提供了新的见解.

关键词:

布拉斯卡纳普斯 (Brassica napus) 是一种植物.这就是Hi-C.在 QTL 精细映射中.基因组结构的变化种子油含量种子油含量

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