整个幻灯片成像 (WSI) 扫描仪的差异影响数字化前列腺癌组织学的光学和计算特性
Savannah R Duenweg1, Samuel A Bobholz2, Allison K Lowman2
1Departments of Biophysics, Medical College of Wisconsin, 8701 Watertown Plank Rd., Milwaukee, WI 53226, USA.
Journal of pathology informatics
|July 27, 2023
概括
数字病理学幻灯片扫描仪在前列腺癌分析中显著影响计算的病态特征. 扫描仪的差异会影响图像属性,因此需要协调,以便可靠的研究和临床使用.
科学领域:
- 数字病理学数字病理学
- 计算病理学计算病理学
- 组织病理学成像成像
背景情况:
- 数字病理学可以实现远程幻灯片分析和协作.
- 幻灯片扫描仪的变化 (分辨率,对比度) 可以影响图像处理结果.
- 标准化对于可复制的病态特征提取至关重要.
研究的目的:
- 为了测试不同的幻灯片扫描仪是否会产生前列腺癌计算病态特征的变化,整体安装幻灯片.
- 评估扫描仪可变性对数字病理学定量图像分析的影响.
主要方法:
- 使用3种不同的扫描仪 (尼康,奥林巴斯,休伦) 数字化了192张H&E染色的前列腺癌幻灯片.
- 应用色彩解卷以标准化图像和计算分段组织.
- 计算了第一和第二阶段的病态特征 (例如细胞密度,光圈面积) 并在扫描仪中进行了比较.
主要成果:
- 扫描仪之间的平均色通道强度 (RGB) 显著不同 (P<.001).
- 血素通道强度在所有扫描仪中都是相似的 (P>.05).
- 大多数病态特征在扫描仪之间有显著差异,除了在特定比较中的光层/层层密度和上皮细胞密度;光层和上皮层面积也存在差异.
结论:
- 幻灯片扫描仪的差异明显影响数字化组织学的光学和计算特性.
- 需要进一步的研究来确定影响细分的特定扫描器属性.
- 协调技术的发展对于数字病理学中跨扫描器数据的比较至关重要.
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