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miR-1183的作用:通过调节分离因子SRSF1来抑制肝细胞癌的潜在抑制剂

Zhilu Yao^1,2, Ning Liu³, Hui Lin¹

¹Department of Gastroenterology, Jingan District Zhabei Central Hospital, Shanghai, 200072, People's Republic of China.

Journal of hepatocellular carcinoma

|July 27, 2023

概括

微RNA-1183 (miR-1183) 通过抑制SRSF1.1,在肝细胞癌 (HCC) 中起到瘤抑制作用. 调 miR-1183 可能为HCC治疗提供一种新的治疗策略.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

肝细胞癌 (HCC) 是一个主要的全球健康问题.
富含氨酸和氨酸的剪接因子1 (SRSF1) 与HCC瘤发生有关.
微RNAs (miRNAs) 在转录后调节基因表达.

研究的目的:

研究miR-1183在肝细胞癌中的作用.
阐明miR-1183影响HCC进展的机制.
评估miR-1183作为HCC的潜在治疗标.

主要方法:

定量逆转录-聚合酶连锁反应 (qRT-PCR) 用于基因表达分析.
在体外测试 (CCK8,殖民地形成,伤口愈合) 来评估细胞功能.
路西法酶记者测定,西部斑点和异种移植瘤测定以确定分子机制和体内效应.
免疫组织化学 (IHC) 用于蛋白质表达分析.

主要成果:

发现miR-1183在HCC细胞系中的下调.
在体外,miR-1183的过度表达减少了HCC细胞的增殖和迁移.
在体内,miR-1183抑制了瘤的形成.
miR-1183直接针对SRSF1,抑制其蛋白质水平.

结论:

miR-1183通过向SRSF1.1,在HCC中起到瘤抑制作用.
这项研究提供了miR-1183通过SRSF1抑制在HCC中的瘤抑制作用的第一个证据.
miR-1183代表了HCC预后和治疗的有前途的治疗标.

关键词:

在HCC中,HCC是HCC.在SRSF1中,我们可以使用SRSF1.在 miR-1183 的位置上.移民移民迁移迁移它们的扩散和扩散.

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