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Updated: Jul 21, 2025

Structure of HIV-1 Capsid Assemblies by Cryo-electron Microscopy and Iterative Helical Real-space Reconstruction
12:38

Structure of HIV-1 Capsid Assemblies by Cryo-electron Microscopy and Iterative Helical Real-space Reconstruction

Published on: August 9, 2011

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蛋白质结构预测,原子模型构建和验证使用B型肝炎病毒球形亚病毒粒子的冷EM密度图

Nadia DiNunno1, Emily N Bianchini1, Haitao Liu1

  • 1Department of Microbiology and Immunology, The Pennsylvania State University College of Medicine, Hershey, PA 17033, USA.

Bio-protocol
|July 27, 2023
PubMed
概括

研究人员使用冷电子显微镜以6.3 Å的精度解决了乙型肝炎病毒 (HBV) 球形亚病毒粒子 (SVP) 结构. 一个新的协议将AlphaFold2与冷EM数据相结合,用于可靠的3DHBV SVP建模.

关键词:
在AlphaFold2中,我们使用了AlphaFold2.化电磁波是一种冷电磁波.这是一种HBV HBV.这就是HBsAg.模型建筑模型建筑高级副总裁SVP

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Determination of Molecular Structures of HIV Envelope Glycoproteins using Cryo-Electron Tomography and Automated Sub-tomogram Averaging07:29

Determination of Molecular Structures of HIV Envelope Glycoproteins using Cryo-Electron Tomography and Automated Sub-tomogram Averaging

Published on: December 1, 2011

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Cryo-electron Microscopy01:28

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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The primary structure of a protein is its amino acid sequence....
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Protein Complex Assembly

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Viruses are extraordinarily diverse in shape and size, but they all have several structural features in common. All viruses have a core that contains a DNA- or RNA-based genome. The core is surrounded by a protective coat of proteins called the capsid. The capsid is composed of subunits called capsomeres. The capsid and genome-containing core are together known as the nucleocapsid.
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