在现场的染色体-RNA反转录序列 (CRIST-seq) 方法对非编码RNA交互网络进行分析
Shilin Zhang1,2, Xue Wen1, Lei Zhou1
1Key Laboratory of Organ Regeneration and Transplantation of Ministry of Education, Cancer Center, First Hospital of Jilin University, Changchun, Jilin 130061, China.
Bio-protocol
|July 27, 2023
概括
我们开发了CRIST-seq,这是一种识别所有调节特定基因的非编码RNA (ncRNA) 的新方法. 这种技术对基因调节网络进行目录,为任何感兴趣的基因推进表观遗传学研究.
科学领域:
- 分子生物学分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 基因组学就是基因组学.
背景情况:
- 非编码RNAs (ncRNAs) 调节基因表达,在生理和病理过程中至关重要.
- 目前用于识别调节性ncRNA的方法是有限的,因为它们不能捕获针对特定基因的所有ncRNA.
- 了解ncRNA参与基因调节对于解开复杂的生物机制至关重要.
研究的目的:
- 引入一种新的方法,CRIST-seq,用于全面识别与特定基因调节相关的ncRNA.
- 通过准Sox2基因,对参与维持多能性的ncRNA调节网络进行目录.
- 为研究整个基因组的表观遗传调节提供一种普遍适用的方法.
主要方法:
- 在现场逆转录序列化 (CRIST-seq) 染色体-RNA的发展.
- 使用CRISPR-dCas9 gRNAs准特定的基因 (例如多能基因Sox2).
- 编目与目标基因调节区域相关的ncRNA.
主要成果:
- CRIST-seq成功地识别了与目标基因调节相关的所有ncRNA.
- 这项研究对ncRNA调节网络进行了目录,该网络对于维持多能性至关重要,以Sox2基因为中心.
- 证明了CRIST-seq的普遍适用性,用于研究任何基因的表观遗传调节.
结论:
- CRIST-seq提供了一种强大的新技术,用于选调节性ncRNA和建立染色体相互作用网络.
- 该方法可适应任何感兴趣的基因和任何基因组网站.
- CRIST-seq可以扩展到检测与RNA,DNA和蛋白质的相互作用,在分子生物学研究中提供广泛的应用.
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