相关实验视频

Updated: Jul 21, 2025

Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis

Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis

Published on: December 12, 2019

利基细胞调节原始生殖细胞静止,以响应底层膜信号

Daniel C McIntyre^1,2,3, Jeremy Nance^1,2

¹Skirball Institute of Biomolecular Medicine, NYU Grossman School of Medicine, New York, NY 10016, USA.

Development (Cambridge, England)

|July 27, 2023

概括

底层膜 (BM) 间接调节干细胞静止. 细胞将BM信号传递给干细胞,通过整合素受体控制它们的增殖.

科学领域:

细胞生物学细胞生物学
发展生物学发展生物学
细胞外矩阵生物学细胞外矩阵生物学

背景情况:

干细胞的命运 (静止,增殖,分化) 是由利基细胞和底层膜 (BM) 相互作用决定的.
虽然直接的BM接触调节了干细胞静止,但间接信号机制仍然不太了解.
在Caenorhabditis elegans中,原始生殖细胞 (PGC) 作为研究干细胞行为间接BM调节的模型.

研究的目的:

研究基底膜 (BM) 如何调节Caenorhabditis elegans原始生殖细胞 (PGC),这些细胞不会直接接触到BM.
阐明参与PGC静止和扩散的间接BM调节的信号通路.

主要方法:

拉米宁的耗尽,以观察PGC的扩散.
分析珀莱干在拉米林耗尽后PGC扩散中的作用.
调查整合素受体参与从BM传递信号到利基细胞的信号中继.
评估幼虫中珀莱肯水平,以评估生理学相关性.

主要成果:

在平时静止的胚胎PGC中,拉米宁耗尽诱导了增殖,这表明BM在维持静止中的作用.
有证据表明,在拉米宁耗尽后,细胞通过整合素受体将从淋巴体BM传递的增殖信号传递给PGC.
珀莱肯被确定为一个关键的调解者;当拉米宁耗尽时,其破坏阻止了PGC的扩散,这表明拉米宁抑制了来自珀莱肯的信号.
降低的perlecan水平损害了幼虫的生殖线生长,支持BM信号在生殖细胞增殖中的生理作用.

结论:

底层膜 (BM) 通过细胞间接调节干细胞静止和增殖.
利基细胞上的整合素受体对于将BM衍生的信号传递给干细胞至关重要.
在BM中,珀莱干和拉米因在控制诱导增殖信号方面起着相反的作用,而拉米因则起着抑制作用.

关键词:

原蛋白是一种原蛋白.整体的整体是整体的拉米尼尼的拉米尼尼.利基市场是一个利基.珀莱莱肯 (Perlecan) 是一个巨人.原始的生殖细胞.

更多相关视频

Dissection and Staining of Drosophila Larval Ovaries

Dissection and Staining of Drosophila Larval Ovaries

Published on: May 13, 2011

Using Primary Neurosphere Cultures to Study Primary Cilia

Using Primary Neurosphere Cultures to Study Primary Cilia

Published on: April 14, 2017

相关概念视频

Stem Cell Niche

Stem Cell Niche

The stem cell niche is the dynamic microenvironment where stem cells reside. Inside these niches, the cells may remain undifferentiated, undergo high self-renewal, or become lineage-specific progenitors. Stem cells coexist with other niche cells, such as stromal cells. They also interact closely with the ECM. Cell-cell and cell-matrix communication occur via adhesion molecules or soluble factors that signal the stem cells and determine their fate. Stromal cells also provide survival signals to...

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...

Multipotency of Hematopoietic Stem Cells

Multipotency of Hematopoietic Stem Cells

The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...

Maintenance of the ES Cell State

Maintenance of the ES Cell State

The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...

Notch Signaling Pathway

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...

Hedgehog Signaling Pathway

Hedgehog Signaling Pathway

The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...