相关实验视频

Updated: Jul 21, 2025

A Fast and Reliable Pipeline for Bacterial Transcriptome Analysis Case study: Serine-dependent Gene Regulation in Streptococcus pneumoniae

A Fast and Reliable Pipeline for Bacterial Transcriptome Analysis Case study: Serine-dependent Gene Regulation in Streptococcus pneumoniae

Published on: April 25, 2015

一种转录基因方法来了解患者在腹部手术后对肺炎的敏感性

Hew D Torrance¹, Ping Zhang^2,3, E Rebecca Longbottom⁴

¹Division of Anaesthetics, Pain Medicine & Intensive Care Department of Surgery & Cancer, Faculty of Medicine, Imperial College London, London. UK.

Annals of surgery

|July 27, 2023

概括

大型腹部手术改变了免疫反应,增加了天生的免疫力,抑制了适应性免疫力. 手术前的中性粒细胞基因特征和术后的败血症反应得分可以预测手术患者的肺炎风险.

科学领域:

免疫学免疫学免疫学
基因组学就是基因组学.
手术并发症手术并发症

背景情况:

手术后的肺炎是主要腹部手术患者死亡的重要原因.
确定感染风险的预测生物标志物对于患者管理至关重要.

研究的目的:

在重大腹部手术后,描述免疫路径和基因网络的改变.
为了识别可预测术后肺炎的转录学模式.

主要方法:

全血RNA测序对150名在多个术后时间点接受重大腹部手术的患者进行了测序.
对患有肺炎的患者进行了转录组分析,与没有感染的患者相比.

主要成果:

手术诱导了显著的基因表达变化,包括先天性免疫激活和适应性免疫抑制.
手术前的中性粒细胞降粒标志和手术后的败血症反应标志 (SRSq) 评分预测了肺炎的发展.
这些签名在独立的外科手术和创伤队伍中显示出预测价值.

结论:

大型腹部手术对免疫路径产生深远的影响,在患有肺炎的患者中观察到不同的模式.
术前和术后早期的转录基因特征对预测肺炎风险具有前景.

更多相关视频

Efficient Transcriptionally Controlled Plasmid Expression System for Investigation of the Stability of mRNA Transcripts in Primary Alveolar Epithelial Cells

Efficient Transcriptionally Controlled Plasmid Expression System for Investigation of the Stability of mRNA Transcripts in Primary Alveolar Epithelial Cells

Published on: March 6, 2020

Murine Oropharyngeal Aspiration Model of Ventilator-associated and Hospital-acquired Bacterial Pneumonia

Murine Oropharyngeal Aspiration Model of Ventilator-associated and Hospital-acquired Bacterial Pneumonia

Published on: June 28, 2018

相关概念视频

Pneumonia I: Introduction

Pneumonia I: Introduction

Pneumonia is an acute respiratory infection that targets the lungs, specifically the alveoli. These tiny air sacs, essential for oxygen exchange, become engorged with pus and fluid, severely hindering breathing, decreasing oxygen absorption, and causing significant pain and discomfort during respiration.
Risk Factors
Various factors influence the likelihood of developing pneumonia. Age plays a crucial role, with infants, children under two, and individuals over 65 at increased risk due to their...

Pneumonia II: Pathophysiology

Pneumonia II: Pathophysiology

The pathophysiology of pneumonia involves the following steps:

Pneumonia III: Complications and Assessment

Pneumonia III: Complications and Assessment

Pneumonia poses the potential for numerous complications that warrant consideration. These complications include the following: