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快速回暖芯片的研究,通过焦尔加热进行冷保存

Hengxin Han^1,2,3, Taijie Zhan^1,2,3, Mengdong Cui^1,2,3

¹Institute of Biothermal Science & Technology, University of Shanghai for Science and Technology, Shanghai 200093, China.

Langmuir : the ACS journal of surfaces and colloids

|July 27, 2023

概括

一个新的电加热芯片 (EHC) 提供了快速,均的冷保存加热,提高了细胞活力和样本完整性,无需复杂的设备或纳米粒子. 这种朱尔加热方法提高了冷保存的效率和安全性.

科学领域:

生物技术是生物技术.
低温生物学低温生物学
材料科学材料科学材料科学

背景情况:

快速和均的回暖对于成功的冷保存至关重要.
使用纳米粒子激光或无线电频率的现有方法是复杂的,并引发生物安全问题.
目前的技术在不同样本体积的高效传热方面存在困难.

研究的目的:

开发一种简单,高效和安全的方法,用于快速冷保存再加热.
为了研究基于朱尔加热的电加热芯片 (EHC) 对于冷保存的有效性.
评估EHC介导的回暖对细胞活力和结构完整性的影响.

主要方法:

基于朱尔加热的电加热芯片 (EHC) 的设计和制造.
测试不同量的冷保护剂 (CPA) 溶液的重新加热速度.
使用人类肺腺癌 (A549) 细胞和人类介质干细胞 (MSCs) 验证冷保存疗效.
细胞活力,结构完整性,亡和亡后冷保存的评估.

主要成果:

该EHC实现了异常高的加热率 (高达3.2 × 10^5 °C/分钟0.28毫升CPA).
重温速度大约比传统方法高出两倍.
与冷瓶相比,降低了超冷却和提高了细胞活力 (A549细胞的97.2%,MSC的93.18%)
增强球状体的结构完整性和减少解后的亡/亡.

结论:

基于焦耳加热的EHC提供了高效,快速和均的冷保存再加热解决方案.
这种方法克服了现有技术的局限性,提供了更好的安全性和有效性.
EHC显著提高了冷保存的细胞和球体的质量和数量,推进了冷保存协议.

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