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无针注射系统的新用途,用于改善核酸的输送和表达in vivo

Nathan Liu^1,2, Hani Abd-Ul-Salam^2,3,4, Noémie Joannette-Lafrance^1,2

¹Faculty of Medicine & Health Sciences, McGill University, Montreal, Quebec, H3G 2M1, Canada.

|July 27, 2023

概括

这项研究引入了使用MED-JET H4 MULTIJET装置的无针基因传递方法. 与传统的基于针的方法相比,它实现了15倍的传染效率提高.

科学领域:

生物技术是生物技术.
基因治疗基因治疗
分子生物学分子生物学

背景情况:

非病毒性核酸输送,称为传染,通常在体内表现出有限的疗效.
目前基于针的体内传染方法可能对患者来说是侵入性的和不舒服的.

研究的目的:

通过使用MED-JET H4 MULTIJET (MJH4M) 装置评估一种新的非侵入性in vivo基因传递协议.
为了比较这种无针针系统的透效率与传统的以针针为基础的输送.

主要方法:

使用无针MED-JET H4 MULTIJET (MJH4M) 装置进行体内基因传递.
采用了基于葡萄糖的"自制"和商业转染试剂.
将MJH4M装置与标准的基于针的输送方法之间的转染结果进行比较.

主要成果:

没有针的MJH4M装置显示,相对于基于针的方法,传染效率显著增加了15倍.
当使用5%的葡萄糖溶液作为输送载体时,可以达到最佳的传染效率.

结论:

无针的MJH4M装置提供了一个高效和非侵入性的替代方案,用于in vivo基因传递.
基于葡萄糖的溶液,特别是5%的葡萄糖,可以作为有效的载体,以提高这种无针技术的传染效率.

关键词:

一个注射的注射.光酶是一种光酶.没有针的,没有针的.核酸的输送核酸的输送转化过程中的转化.

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