循环RNA_0000326通过调节miR-9-3p/YAP1轴加速乳腺癌的发展
Xiao-Ling Xue1, Shuai Zhao2, Meng-Chang Xu3
1Radiology Department, Fujian Maternity and Child Health Hospital, Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian, China.
Neoplasma
|July 27, 2023
概括
循环RNA_0000326通过调节microRNA-9-3p/Yes相关蛋白1轴促进乳腺癌 (BC) 的发展. 沉默这个circRNA可以抑制BC细胞的生长,入侵和瘤的进展.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 循环RNA (circRNA)_0000326与膀和宫癌有关.
- 它在乳腺癌 (BC) 发展中的作用仍然未被探索.
- 了解BC中的circ_0000326机制对于向治疗至关重要.
研究的目的:
- 研究circ_0000326在乳腺癌中的表达和功能作用.
- 阐明circ_0000326对BC进展的影响背后的分子机制.
- 探索circ_0000326作为BC的一个治疗点的潜力.
主要方法:
- 定量实时PCR (RT-qPCR) 来评估BC组织和细胞中的circ_0000326表达.
- 细胞测试 (CCK-8,流细胞计,Transwell,球体形成) 来评估circ_0000326,microRNA-9-3p (miR-9-3p) 和Yes相关蛋白1 (YAP1) 对BC细胞行为的影响.
- 生物信息分析 (starBase),露西法酶记者测定和皮尔森的相关性来确认circ_0000326,miR-9-3p和YAP1.1之间的相互作用.
- 在体内皮下瘤发生模型,以评估circ_0000326沉默对瘤生长的影响.
主要成果:
- 在BC组织和细胞中,circ_0000326显著上调,与预后不佳相关.
- 沉默circ_0000326抑制了BC细胞的增殖,入侵和干性,降低了SOX2和OCT4蛋白水平.
- circ_0000326作为miR-9-3p的分子海绵,这反过来又针对YAP1,揭示了一个circ_0000326/miR-9-3p/YAP1调节轴.
- 在体内实验表明,circ_0000326沉默抑制了BC瘤的生长.
结论:
- circ_0000326促进乳腺癌的发展和进展.
- 该机制涉及circ_0000326/miR-9-3p/YAP1通路,影响细胞生长,入侵和茎状.
- circ_0000326 是乳腺癌的潜在诊断生物标志物和治疗点.
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